Triebel F, Breathnach R, Graziani M, Hercend T, Debre P
Laboratoire d'Immunologie Cellulaire, Institut Gustave-Roussy, Villejuif, France.
J Immunol. 1988 Jan 1;140(1):300-4.
We have cloned and sequenced the human T cell receptor alpha- and beta-chains cDNA present in a lambda gt10 library derived from a human CD4+ diphtheria toxoid-specific T cell clone, PH28. Two 1.3-kb beta-transcripts within frame V, D, J joining were found. These two functional beta-transcripts use two members of different V beta-families, V beta 3 and V beta 4, one joined to D beta 1-1, J beta 1-1, C beta 1, the other joined to D beta 1-1, J beta 2-1, C beta 2. Moreover, we have cloned the two different V beta germ-line counterparts whose sequences confirm that these two V beta are not pseudogenes. Analysis of T cell receptor beta and alpha rearrangements in PH28 showed that a single rearrangement on each chromosome could be assigned either at the J beta or the J gamma loci. These results strongly suggest an absence of allelic exclusion for 1.3-kb mRNA beta-transcripts in a human antigen-specific T cell clone.
我们已经克隆并测序了存在于来自人类CD4 + 白喉类毒素特异性T细胞克隆PH28的λgt10文库中的人类T细胞受体α链和β链cDNA。在框架V、D、J连接中发现了两个1.3kb的β转录本。这两个功能性β转录本使用不同Vβ家族的两个成员，Vβ3和Vβ4，一个与Dβ1-1、Jβ1-1、Cβ1连接，另一个与Dβ1-1、Jβ2-1、Cβ2连接。此外，我们克隆了两个不同的Vβ种系对应物，其序列证实这两个Vβ不是假基因。对PH28中T细胞受体β和α重排的分析表明，每条染色体上的单个重排可以指定在Jβ或Jγ基因座。这些结果强烈表明在人类抗原特异性T细胞克隆中，1.3kb mRNAβ转录本不存在等位基因排斥。
