一个抗原特异性T细胞克隆表达两种不同的T细胞受体α/β异二聚体。
Expression of two distinct T cell receptor alpha/beta heterodimers by an antigen-specific T cell clone.
作者信息
Matis L A, Ezquerra A, Coligan J E
机构信息
Division of Biochemistry and Biophysics, Food and Drug Administration, Bethesda, Maryland.
出版信息
J Exp Med. 1988 Dec 1;168(6):2379-84. doi: 10.1084/jem.168.6.2379.
Abstract
A cytochrome c-specific, MHC-restricted T cell clone with two complete rearrangements of the same V beta 1 gene element was shown to express two different TCR alpha/beta heterodimers. Antipeptide antisera specific for TCR C beta 1 and C beta 2 peptides each immunoprecipitated distinct disulfide-linked cell surface heterodimers. The clone was derived from immunized allogeneic chimeric mice, and displayed multiple Ia specificities, including the ability to recognize antigen in association with both I-Ek and I-Es Ia molecules, as well as alloreactivity to the I-Eb molecule. It will be important to determine whether each receptor contributes independently to the overall specificity of the clone.
摘要
一个细胞色素c特异性、MHC限制的T细胞克隆，其相同的Vβ1基因元件有两次完整重排，被证明表达两种不同的TCRα/β异二聚体。针对TCR Cβ1和Cβ2肽的抗肽抗血清分别免疫沉淀出不同的二硫键连接的细胞表面异二聚体。该克隆源自免疫的同种异体嵌合小鼠，表现出多种Ia特异性，包括识别与I-Ek和I-Es Ia分子相关的抗原的能力，以及对I-Eb分子的同种异体反应性。确定每个受体是否独立地对克隆的整体特异性起作用将很重要。
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