Ding Nannan, Luo Shasha, Yu Jinjin, Zhou Ying, Wu Zhifeng
Department of Ophthalmology, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, China.
Mol Vis. 2018 Mar 26;24:252-260. eCollection 2018.
This study aims to quantify the concentration of apolipoprotein A1 (APOA1) and retinol binding protein (RBP4) expressed in the vitreous humors of patients with rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD), rhegmatogenous retinal detachment (RRD), and idiopathic epimacular membrane (IEM). This study also aims to investigate the potential role of APOA1 and RBP4 as biomarkers of RRDCD.
Enzyme-linked immunosorbent assay (ELISA) kits were used to obtain levels of APOA1 and RBP4 from the vitreous humor samples of 76 primary patients. These patients included 23 patients with RRDCD, 28 patients with RRD, and 24 patients with IEM. All patients were undergoing planned pars plana vitrectomy. The differences between the concentrations of the molecular biomarkers among different patient groups were analyzed using the Mann-Whitney U-test for nonparametric values and independent samples test or one-way ANOVA analysis for parametric data. The relationship between the molecular biomarkers, grades of proliferative vitreoretinopathy (PVR), and quadrants of retinal detachment were analyzed using nonparametric Spearman's rank correlation analysis.
The vitreous concentrations of APOA1 and RBP4 were statistically significantly higher in the RRDCD group compared to the RRD and IEM groups. Patients with severe PVR demonstrated a higher concentration of APOA1 and RBP4 compared to those with mild PVR, but this finding was not statistically significant. There was a statistically significant positive correlation between APOA1 and RBP4 in the RRDCD and RRD groups. Nonparametric Spearman's rank correlation analysis revealed that levels of APOA1 and RBP4 increased statistically significantly with an increasing number of detached retinal quadrants in the RRDCD and RRD groups.
The findings of this study allude to the potential of APOA1 and RBP4 as specific biomarkers of RRDCD. The findings of this study may contribute to increased understanding regarding the role of APOA1 and RBP4 in RRDCD.
本研究旨在量化孔源性视网膜脱离合并脉络膜脱离（RRDCD）、孔源性视网膜脱离（RRD）和特发性黄斑前膜（IEM）患者玻璃体液中载脂蛋白A1（APOA1）和视黄醇结合蛋白（RBP4）的浓度。本研究还旨在探讨APOA1和RBP4作为RRDCD生物标志物的潜在作用。
使用酶联免疫吸附测定（ELISA）试剂盒从76例原发性患者的玻璃体液样本中获取APOA1和RBP4的水平。这些患者包括23例RRDCD患者、28例RRD患者和24例IEM患者。所有患者均计划接受玻璃体切割术。使用非参数值的曼-惠特尼U检验以及参数数据的独立样本检验或单因素方差分析来分析不同患者组之间分子生物标志物浓度的差异。使用非参数斯皮尔曼等级相关分析来分析分子生物标志物、增殖性玻璃体视网膜病变（PVR）分级和视网膜脱离象限之间的关系。
与RRD组和IEM组相比，RRDCD组玻璃体液中APOA1和RBP4的浓度在统计学上显著更高。与轻度PVR患者相比，重度PVR患者的APOA1和RBP4浓度更高，但这一发现无统计学意义。RRDCD组和RRD组中APOA1和RBP4之间存在统计学上显著的正相关。非参数斯皮尔曼等级相关分析显示，RRDCD组和RRD组中，随着视网膜脱离象限数量的增加，APOA1和RBP4的水平在统计学上显著升高。
本研究结果表明APOA1和RBP4有可能作为RRDCD的特异性生物标志物。本研究结果可能有助于加深对APOA1和RBP4在RRDCD中作用的理解。
