Department of Ophthalmology, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, People's Republic of China.
Department of Ophthalmology, Affiliated Wuxi Clinical College of Nantong University, Wuxi, People's Republic of China.
Curr Eye Res. 2022 May;47(5):809-812. doi: 10.1080/02713683.2022.2038634. Epub 2022 Apr 11.
This study aims to explore the differences in the levels of complement components and complement regulatory factors in the vitreous humor of patients with retinal detachment with choroidal detachment (RRDCD), patients with rhegmatogenous retinal detachment (RRD).
A prospective case-control study design was used to recruit 20 patients with RRDCD and 20 patients with RRD in consecutive cases who underwent pars plana vitrectomy from March 2019 to January 2020. The control group comprised 15 patients with epiretinal membrane and 5 eyes from cadavers. The concentrations of complement C2, complement C4b, complement C5/C5a, complement C9, complement factor D (CFD), lectin, and complement factor I (CFI) were measured using Multiplex Luminex Assay, and the concentration of soluble decay acceleration factor (sDAF) was measured using ELISA.
As compared with the RRD and control groups, complement C2, complement C4b, complement C5/C5a, complement C9, CFD, lectin, CFI, and sDAF were significantly increased in the RRDCD group. Additionally, as compared with the control group, the concentrations of complement component C2 and CFD were significantly increased in the vitreous humor of the RRD group.
Components of all three complement pathways were elevated in eyes with RRDCD. Interestingly, while there was evidence of early complement activation in RRD, the final common pathway components were not elevated. In contrast, RRDCD eyes showed significant elevations of the MAC complex components, underscoring a potential pathophysiologic impact of complement activation in this condition.
本研究旨在探讨伴有脉络膜脱离的视网膜脱离（RRDCD）患者和孔源性视网膜脱离（RRD）患者玻璃体内补体成分和补体调节因子水平的差异。
采用前瞻性病例对照研究设计，连续纳入 2019 年 3 月至 2020 年 1 月期间接受经睫状体平坦部玻璃体切除术的 20 例 RRDCD 患者和 20 例 RRD 患者。对照组包括 15 例眼内膜病变患者和 5 例尸眼。采用多重 Luminex assay 检测补体 C2、补体 C4b、补体 C5/C5a、补体 C9、补体因子 D（CFD）、凝集素和补体因子 I（CFI）的浓度，采用 ELISA 法检测可溶性衰变加速因子（sDAF）的浓度。
与 RRD 和对照组相比，RRDCD 组补体 C2、补体 C4b、补体 C5/C5a、补体 C9、CFD、凝集素、CFI 和 sDAF 显著升高。此外，与对照组相比，RRD 组玻璃体内补体成分 C2 和 CFD 的浓度显著升高。
RRDCD 眼的所有三条补体途径的成分均升高。有趣的是，虽然 RRD 存在早期补体激活的证据，但最终共同途径的成分并未升高。相比之下，RRDCD 眼显著升高了 MAC 复合物成分，这强调了补体激活在这种情况下可能具有潜在的病理生理影响。
