印度尼西亚泗水苏托莫医院宫颈上皮内瘤变和鳞状细胞癌中人乳头瘤病毒的基因分型
GENOTYPING OF HUMAN PAPPILOMAVIRUS IN CERVICAL PRECANCEROUS LESION AND SQUAMOUS CELL CARCINOMA AT DR. SOETOMO HOSPITAL, SURABAYA, INDONESIA.
作者信息
Mastutik Gondo, Alia Rahmi, Rahniayu Alphania, Rahaju Anny Setijo, Kurniasari Nila, Putra Suhartono Taat
机构信息
Department of Anatomic Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya 60131, Indonesia.
Prof Dr Soekandar Hospital, Mojokerto 61318, Indonesia.
出版信息
Afr J Infect Dis. 2018 Mar 7;12(1 Suppl):7-12. doi: 10.21010/Ajid.12v1S.2. eCollection 2018.
BACKGROUND
Cervical cancer caused by human papilloma virus (HPV), is the second most common cancer for women. This cancer is distributed worldwide, with ~80% of cases are found in the developing countries. In Indonesia, data of HPV genotypes are still limited and do not represent all regions of the country. Thus, here we report genotyping of HPV samples collected from the Dr. Soetomo Hospital Surabaya Indonesia patients, in 2013.
MATERIALS AND METHOD
A cross sectional study was performed using 68 paraffin blocks of low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC) cervix.
RESULT
This study showed that HPV genotypes found in LSIL samples are HPV 16, 18, 6/33 or 68/72. Furthermore, those in HSIL are HPV 16, 18, 52, 59, 67, 6/18, 6/45, 16/67, 26/61, or 52/67, while in SCC are HPV 16, 18, 45, 52, 56, 16/18 or 16/45. Single-genotype infection, . by HPV 16, 18, 45, 52, 56, 59, or 67, was observed in 86.77% (59/68) of samples, whereas multiple-genotype infections, . by HPV 6/18, 6/33, 6/45, 16/18, 16/45, 16/67, 26/61, 52/67, or 68/72, was found in 13.23% (9/68) of the samples.
CONCLUTIONS
The mostly HPV genotype identified in this study is HPV 16 (62.68%), then followed by HPV 18 (20.9%), HPV 45 (5.97%), 52 (5.97%), and 67 (4.48%). HPV 16 and 18 have used as vaccine, and HPV 45 has cross reaction with HPV 18, then HPV 52 and 67 should be considered as the second-generation HPV vaccines.
背景
人乳头瘤病毒（HPV）引起的宫颈癌是女性第二常见的癌症。这种癌症在全球范围内分布，约80%的病例在发展中国家被发现。在印度尼西亚，HPV基因型的数据仍然有限，且不能代表该国所有地区。因此，在此我们报告2013年从印度尼西亚泗水苏托莫博士医院患者中收集的HPV样本的基因分型。
材料与方法
采用68个低级别鳞状上皮内病变（LSIL）、高级别鳞状上皮内病变（HSIL）和宫颈鳞状细胞癌（SCC）的石蜡块进行横断面研究。
结果
本研究表明，在LSIL样本中发现的HPV基因型为HPV 16、18、6/33或68/72。此外，HSIL中的基因型为HPV 16、18、52、59、67、6/18、6/45、16/67、26/61或52/67，而SCC中的基因型为HPV 16、18、45、52、56、16/18或16/45。86.77%（59/68）的样本观察到单一基因型感染，即由HPV 16、18、45、52、56、59或67引起，而13.23%（9/68）的样本发现多重基因型感染，即由HPV 6/18、6/33、6/45、16/18、16/45、16/67、26/61、52/67或68/72引起。
结论
本研究中鉴定出的最常见HPV基因型是HPV 16（62.68%），其次是HPV 18（20.9%）、HPV 45（5.97%）、52（5.97%）和67（4.48%）。HPV 16和18已被用作疫苗，且HPV 45与HPV 18有交叉反应，那么HPV 52和67应被视为第二代HPV疫苗。
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