Salehi-Vaziri Mostafa, Sadeghi Farzin, Hashemi Firoozeh Sadat, Haeri Hayedeh, Bokharaei-Salim Farah, Monavari Seyed Hamidreza, Keyvani Hossein
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran; Department of Immunology and Microbiology, School of Medicine, Babol University of Medical Sciences, Babol, IR Iran.
Iran Red Crescent Med J. 2016 Jan 31;18(4):e24458. doi: 10.5812/ircmj.24458. eCollection 2016 Apr.
Persistent infection with high-risk human papillomavirus (HPV) has been recognized as a major cause of cervical cancer. Distribution of HPV genotypes may differ according to the geographic region and the severity of the cervical lesion. Determining HPV genotypes' specific distribution is useful for HPV surveillance and control programs. However, little is known about the distribution of HPV genotypes in Iranian women.
The aim of this study was to determine the distribution of HPV genotypes in Iranian women with different grades of cervical lesions.
From 2011 to 2013, a total of 436 Iranian women with convenience sampling strategy were included in this cross-sectional study. In detail, 287 women negative for intraepithelial lesion or malignancy, 32 with atypical squamous cells of undetermined significance (ASCUS), 50 with low-grade squamous intraepithelial lesion (LSIL), 44 with high-grade squamous intraepithelial lesion (HSIL), and 23 with cervical cancer were evaluated in this investigation. HPV genotypes were determined by INNO-LiPA HPV Genotyping Extra assay.
In total, HPV infection was detected in 45.4% of the cases. The most common high-risk HPV (HR-HPV) genotype was HPV-16 (32.8%), followed by HPV-53 (9.1%). Within low-risk (LR-HPV) genotypes HPV-6 (22.2%) and HPV-44 (6.1%) were the most prevalent. HPV-16 was the predominant genotype in cases with cervical cancer (56.5%), ASCUS (34.4%), and HSIL (34.1%). HPV-6 was the most common genotype in normal cases (9.1%) and LSIL patients (18%). The prevalence of HPV positivity was significantly higher in cases with high-grade lesions (≥ HSIL) (64.2%) than in normal/LSIL (37.3%) (P = 0.033). The rate of HR-HPV infection was significantly higher in ≥ HSIL cases (61.2%) than normal/LSIL (27.9%) (P = 0.003).
This study describes robust information on the distribution of HPV genotypes among Iranian women with and without cervical lesions. The present data may be of importance for designing future public health strategies, including HPV vaccination programs.
高危型人乳头瘤病毒（HPV）持续感染已被公认为宫颈癌的主要病因。HPV基因型的分布可能因地理区域和宫颈病变的严重程度而异。确定HPV基因型的具体分布对于HPV监测和防控计划很有用。然而，伊朗女性中HPV基因型的分布情况鲜为人知。
本研究旨在确定不同宫颈病变分级的伊朗女性中HPV基因型的分布情况。
2011年至2013年，本横断面研究采用便利抽样策略纳入了436名伊朗女性。具体而言，本研究评估了287名上皮内病变或恶性肿瘤阴性的女性、32名意义不明确的非典型鳞状细胞（ASCUS）患者、50名低级别鳞状上皮内病变（LSIL）患者、44名高级别鳞状上皮内病变（HSIL）患者以及23名宫颈癌患者。通过INNO-LiPA HPV基因分型额外检测法确定HPV基因型。
总体而言，45.4%的病例检测到HPV感染。最常见的高危型HPV（HR-HPV）基因型是HPV-16（32.8%），其次是HPV-53（9.1%）。在低危型（LR-HPV）基因型中，HPV-6（22.2%）和HPV-44（6.1%）最为常见。HPV-16是宫颈癌（56.5%）、ASCUS（34.4%）和HSIL（34.1%）病例中的主要基因型。HPV-6是正常病例（9.1%）和LSIL患者（18%）中最常见的基因型。高级别病变（≥HSIL）病例中HPV阳性率（64.2%）显著高于正常/LSIL病例（37.3%）（P = 0.033）。≥HSIL病例中HR-HPV感染率（61.2%）显著高于正常/LSIL病例（27.9%）（P = 0.003）。
本研究描述了伊朗有或无宫颈病变女性中HPV基因型分布的有力信息。目前的数据可能对设计未来的公共卫生策略（包括HPV疫苗接种计划）具有重要意义。
