Paling Sepling, Wahyuni Ratna, Winarni Dwi, Astari Linda, Adriaty Dinar, Agusni Indropo, Izumi Shinzo
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia.
Afr J Infect Dis. 2018 Mar 7;12(1 Suppl):44-48. doi: 10.2101/Ajid.12v1S.5. eCollection 2018.
() is a pathogenic bacterium that causes leprosy. The presence of in the environment is supported by microorganisms that act as the new host for . 's potential to be a host of in the environment. sp. is (FLA) that classified as holozoic, saprophytic, and saprozoic. The existence of nutrients in the environment influence ability to phagocytosis or pinocytosis. This study is aimed to determine sp.S-11 phagocytic activity to () which cultured in non-nutrient media and riched-nutrient media.
This research conducted by culturing sp.S-11 and on different nutrient media conditions. intracellular DNA were isolated and amplified by specific primers through Real Time PCR (Q-PCR).
The results showed that co-cultured on non-nutrient media were more active to phagocyte than on rich-nutrient media.
The use of non-nutrient media is recommended to optimize sp. phagocytic activity to .
（某菌名）是一种导致麻风病的致病细菌。环境中该菌的存在得到了作为其新宿主的微生物的支持。（另一菌名）在环境中作为（某菌名）宿主的潜力。（另一菌名）菌属于全动物营养型、腐生型和腐生动物营养型的（某种分类类型）。环境中营养物质的存在会影响（该菌）吞噬作用或胞饮作用的能力。本研究旨在确定（另一菌名）S - 11菌株对在非营养培养基和富营养培养基中培养的（某菌名）的吞噬活性。
本研究通过在不同营养培养基条件下培养（另一菌名）S - 11菌株和（某菌名）来进行。通过实时荧光定量PCR（Q - PCR）使用（某菌名）特异性引物分离并扩增（某菌名）的细胞内DNA。
结果表明，在非营养培养基上共培养的（某菌名）比在富营养培养基上对吞噬（另一菌名）更具活性。
建议使用非营养培养基来优化（另一菌名）菌株对（某菌名）的吞噬活性。
