一种用于检测人性激素结合球蛋白的酶联免疫吸附测定（ELISA）。

An enzyme-linked immunosorbent assay (ELISA) for human sex hormone-binding globulin.

作者信息

Heubner A, Rüde S, Juchem M, Grill H J, Pollow K

机构信息

Abteilung für Experimentelle Endokrinologie, Johannes Gutenberg Universität Mainz, F.R.G.

出版信息

J Steroid Biochem. 1987 Dec;28(6):647-52. doi: 10.1016/0022-4731(87)90393-1.

DOI:10.1016/0022-4731(87)90393-1

PMID:2961947

Abstract

An enzyme-linked immunosorbent assay (ELISA) of the "sandwich-type" for sex hormone binding globulin (SHBG) has been developed. A rabbit anti-SHBG antibody (RAb) is immobilized to the microtitre plate. After incubation with standards and samples a second monospecific rabbit anti-SHBG antibody, labelled with alkaline phosphatase is added (RAb). Following further washing substrate is added, colour developed and the plate read at 405 nm wavelength on a standard ELISA plate reader. The assay is not influenced by the presence of steroids at the binding site, and shows good agreement to SHBG binding capacity assay and commercially available IRMA. Its sensitivity, specificity and precision allows its use in the routine laboratory. The SHBG ELISA has been used to measure SHBG concentrations in sera of normal men, women, pregnant women, and women receiving high-dose medroxyprogesterone acetate as a treatment of metastatic breast cancer.

摘要

已开发出一种用于性激素结合球蛋白（SHBG）的“夹心型”酶联免疫吸附测定（ELISA）法。将兔抗SHBG抗体（RAb）固定在微量滴定板上。与标准品和样品孵育后，加入第二种用碱性磷酸酶标记的单特异性兔抗SHBG抗体（RAb）。进一步洗涤后加入底物，显色，然后在标准ELISA酶标仪上于405nm波长读取平板。该测定不受结合位点处类固醇存在的影响，并且与SHBG结合能力测定和市售免疫放射分析（IRMA）显示出良好的一致性。其灵敏度、特异性和精密度使其可用于常规实验室。SHBG ELISA已用于测量正常男性、女性、孕妇以及接受大剂量醋酸甲羟孕酮治疗转移性乳腺癌的女性血清中的SHBG浓度。