Lewis J G, Elder P A
J Steroid Biochem. 1985 May;22(5):673-6. doi: 10.1016/0022-4731(85)90222-5.
A direct ELISA for plasma cortisol is described which is carried out in a standard 96 well microtitre plate. In this ELISA cortisol-thyroglobulin conjugate is immobilised to the microtitre plate and competes with cortisol in the standard or plasma sample for antibody binding sites. Following washing the rabbit cortisol antibody bound to immobilised cortisol is incubated with peroxidase labelled goat antirabbit IgG. Following further washing o-phenylenediamine is added, colour developed, and the plate read at 492 nm on a standard ELISA plate reader. This ELISA shows good agreement with RIA and its sensitivity, specificity and precision allow its use in the routine steroid laboratory.
本文描述了一种用于血浆皮质醇的直接酶联免疫吸附测定（ELISA），该测定在标准的96孔微量滴定板中进行。在这种ELISA中，皮质醇-甲状腺球蛋白偶联物固定在微量滴定板上，并与标准品或血浆样品中的皮质醇竞争抗体结合位点。洗涤后，将与固定化皮质醇结合的兔皮质醇抗体与过氧化物酶标记的山羊抗兔IgG一起孵育。进一步洗涤后，加入邻苯二胺，显色，然后在标准ELISA酶标仪上于492nm处读取平板。这种ELISA与放射免疫分析（RIA）显示出良好的一致性，其灵敏度、特异性和精密度使其可用于常规类固醇实验室。
