从羊膜、绒毛膜、胎盘蜕膜和脐带中分离人骨髓间充质干细胞：四种酶法方案的比较
Isolation of human mesenchymal stem cells from amnion, chorion, placental decidua and umbilical cord: comparison of four enzymatic protocols.
作者信息
Araújo A B, Furlan J M, Salton G D, Schmalfuss T, Röhsig L M, Silla L M R, Passos E P, Paz A H
机构信息
Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, 90035-903, Porto Alegre, Rio Grande do Sul, Brazil.
Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
Biotechnol Lett. 2018 Jun;40(6):989-998. doi: 10.1007/s10529-018-2546-z. Epub 2018 Apr 4.
OBJECTIVE
To compare four enzymatic protocols for mesenchymal stem cells (MSCs) isolation from amniotic (A-MSC) and chorionic (C-MSC) membranes, umbilical cord (UC-MSC) and placental decidua (D-MSC) in order to define a robust, practical and low-cost protocol for each tissue.
RESULTS
A-MSCs and UC-MSCs could be isolated from all samples using trypsin/collagenase-based protocols; C-MSCs could be isolated from all samples with collagenase- and trypsin/collagenase-based protocols; D-MSCs were isolated from all samples exclusively with a collagenase-based protocol.
CONCLUSIONS
The trypsin-only protocol was least efficient; the collagenase-only protocol was best for C-MSCs and D-MSCs; the combination of trypsin and collagenase was best for UC-MSCs and none of tested protocols was adequate for A-MSCs isolation.
目的
比较四种用于从羊膜（A-MSC）、绒毛膜（C-MSC）、脐带（UC-MSC）和胎盘蜕膜（D-MSC）中分离间充质干细胞（MSC）的酶处理方案，以便为每种组织确定一种可靠、实用且低成本的方案。
结果
使用基于胰蛋白酶/胶原酶的方案可从所有样本中分离出A-MSC和UC-MSC；使用基于胶原酶和胰蛋白酶/胶原酶的方案可从所有样本中分离出C-MSC；仅使用基于胶原酶的方案可从所有样本中分离出D-MSC。
结论
仅用胰蛋白酶的方案效率最低；仅用胶原酶的方案对C-MSC和D-MSC最佳；胰蛋白酶和胶原酶联合使用对UC-MSC最佳，且所测试的方案均不适用于A-MSC的分离。
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