Asgari Hamid Reza, Akbari Mohammad, Yazdekhasti Hossein, Rajabi Zahra, Navid Shadan, Aliakbari Fereshte, Abbasi Niloufar, Aval Freidon Sargolzaei, Shams Alireza, Abbasi Mehdi
1 Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences , Tehran, Iran .
2 Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
Cell Reprogram. 2017 Feb;19(1):44-53. doi: 10.1089/cell.2016.0035.
Placenta harbors a plentiful source of various cells with stem cells or stem-like cell properties, which can be used in therapeutic procedures and research. Mesenchymal stem cells (MSCs) have attracted much attention due to their specific differentiation potential and tolerogenic properties. MSCs have been isolated from different parts of placenta; however, in this study, we isolated MSCs from amnion and chorion membrane, as well as umbilical cord (Wharton's jelly [WJ]) and compared their capacity regarding differentiation toward female germ cells under influence of 10 ng/mL BMP4. All placenta samples were collected from delivering mothers by normal cesarean section and cells were isolated by different methods. Results showed that all isolated cells were mostly positive for the MSC markers CD73, CD166, and CD105, and minimally reacted with CD34 and CD45 (hematopoietic markers). After differentiation induction using third passage cultured cells, immunocytochemistry staining showed that cells were positive for germline cell-related genes Ssea4, Oct4, and Ddx4, and oocyte-related gene Gdf9. RT-qPCR results indicated that human chorion MSCs (hCMSCs) had a greater potential to be differentiated into female germline cells. Moreover, the results of this study indicate that human umbilical cord MSCs originated from either male or female umbilical cord have the same differentiation potential into female germline cells. We recommend that for presumptive application of MSCs for infertility treatment and research, hUMSCs are best candidates due to their higher differentiation potential, ease of proliferation and expansion, and low immunogenicity.
胎盘含有丰富的具有干细胞或干细胞样特性的各种细胞来源，可用于治疗程序和研究。间充质干细胞（MSCs）因其特定的分化潜能和免疫耐受特性而备受关注。MSCs已从胎盘的不同部位分离出来；然而，在本研究中，我们从羊膜和绒毛膜以及脐带（华通氏胶[WJ]）中分离出MSCs，并比较了它们在10 ng/mL骨形态发生蛋白4（BMP4）影响下向雌性生殖细胞分化的能力。所有胎盘样本均通过正常剖宫产从分娩的母亲处采集，细胞通过不同方法分离。结果表明，所有分离的细胞大多对MSCs标志物CD73、CD166和CD105呈阳性，与CD34和CD45（造血标志物）反应极小。使用第三代培养细胞进行分化诱导后，免疫细胞化学染色显示细胞对生殖系细胞相关基因Ssea4、Oct4和Ddx4以及卵母细胞相关基因Gdf9呈阳性。逆转录-定量聚合酶链反应（RT-qPCR）结果表明，人绒毛膜间充质干细胞（hCMSCs）具有更大的分化为雌性生殖系细胞的潜力。此外，本研究结果表明，源自男性或女性脐带的人脐带间充质干细胞（hUMSCs）向雌性生殖系细胞分化的潜能相同。我们建议，对于MSCs在不孕症治疗和研究中的推定应用，hUMSCs是最佳候选者，因为它们具有更高的分化潜能、易于增殖和扩增以及低免疫原性。
