Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
Chin J Integr Med. 2019 Jun;25(6):439-445. doi: 10.1007/s11655-018-2976-4. Epub 2018 Apr 4.
To investigate the protective effects of Shexiang Tongxin Dropping Pill (, STP) on NaSO-induced hypoxia-reoxygenation injury in cardiomyoblast H9c2 cells.
The cell viability and levels of mRNA and protein expression in H9c2 cells were determined following NaSO-induced hypoxia using Hoechst staining, annexin V/propidium iodide (PI) flow cytometry, real-time polymerase chain reaction and Western blot analysis.
STP pretreatment significantly increased the viability and inhibited aberrant morphological changes in H9c2 cardiomyoblast cells induced by NaSO treatment (P<0.05). In addition, STP pretreatment attenuated NaSO-induced hypoxic damage, down-regulated the expression of pro-apoptotic Bax, and up-regulated the expression of anti-apoptotic Bcl-2 in H9c2 cells (P<0.05).
STP was strongly cardioprotective in hypoxia-reoxygenation injury by preventing hypoxic damage and inhibiting cellular apoptosis. These results further support the use of STP as an effective drug for the treatment of ischemic heart disease.
研究麝香通心滴丸（STP）对 NaSO 诱导的心肌细胞 H9c2 缺氧再复氧损伤的保护作用。
采用 Hoechst 染色、 Annexin V/PI 流式细胞术、实时聚合酶链反应和 Western blot 分析检测 NaSO 诱导缺氧后 H9c2 细胞的细胞活力和 mRNA 及蛋白表达水平。
STP 预处理可显著提高 NaSO 处理诱导的 H9c2 心肌细胞活力，抑制异常形态变化（P<0.05）。此外，STP 预处理可减轻 NaSO 诱导的缺氧损伤，下调促凋亡 Bax 的表达，上调抗凋亡 Bcl-2 的表达（P<0.05）。
STP 通过预防缺氧损伤和抑制细胞凋亡对缺氧再复氧损伤具有强烈的心脏保护作用。这些结果进一步支持 STP 作为治疗缺血性心脏病的有效药物。
