Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210038, PR China;
Chin J Cancer Res. 2013 Apr;25(2):183-91. doi: 10.3978/j.issn.1000-9604.2013.02.06.
To study the mechanisms in gambogic acid (GA) -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro.
The proliferation of GA-treated JeKo-1 cells was measured by CCK-8 assay and Ki-67 immunocytochemical detection. Apoptosis, cell cycle and mitochondrial membrane potential were measured by flow cytometric analysis. Caspase-3, -8 and -9 were detected by colorimetric assay. Bcl-2 and Bax were analyzed by Western blotting.
GA inhibited cell growth in a time- and dose- dependent manner. GA induces apoptosis in JeKo-1 cells but not in normal bone marrow cells, which was involved in reducing the membrane potential of mitochondria, activating caspases-3, -8 and -9 and decreasing the ratio of Bcl-2 and Bax without cell cycle arresting.
GA induced apoptosis in human MCL JeKo-1 cells by regulating Bcl-2/Bax and activating caspase-3, -8 and -9 via mitochondrial pathway without affecting cell cycle.
研究藤黄酸（GA）体外诱导 JeKo-1 人套细胞淋巴瘤细胞凋亡的机制。
用 CCK-8 法和 Ki-67 免疫细胞化学检测观察 GA 处理的 JeKo-1 细胞的增殖。用流式细胞术分析检测细胞凋亡、细胞周期和线粒体膜电位。用比色法检测 caspase-3、-8 和 -9。用 Western blot 分析 Bcl-2 和 Bax。
GA 呈时间和剂量依赖性抑制细胞生长。GA 诱导 JeKo-1 细胞凋亡，但不诱导正常骨髓细胞凋亡，其涉及降低线粒体膜电位、激活 caspase-3、-8 和 -9 以及降低 Bcl-2 和 Bax 比值而不影响细胞周期。
GA 通过调节 Bcl-2/Bax 并通过线粒体途径激活 caspase-3、-8 和 -9，而不影响细胞周期，诱导人套细胞淋巴瘤 JeKo-1 细胞凋亡。
