State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Nanoscale. 2018 Apr 19;10(15):7163-7170. doi: 10.1039/c8nr00223a.
As a ubiquitous hydrolysis enzyme in phosphate metabolism, alkaline phosphatase (ALP) is a significant biomarker in laboratory research and clinic diagnosis. Herein, we report a highly water-soluble Eu(DPA)3@Lap nanohybrid material for the rapid and selective assay of PPi and ALP through a luminescence off-on recognition process. Eu(DPA)3@Lap was successfully prepared in an aqueous solution, and it exhibited strong luminescence emission, high photostability, and long lifetime. More interestingly, the strong luminescence of Eu(DPA)3@Lap can be remarkably quenched by Cu2+ due to the high impetus of coordination between the DPA ligand and Cu2+ ion. Using Cu2+ as a signal transducer, the luminescence could be recovered upon the addition of PPi ion owing to the formation of a Cu2+-PPi complex; thus, a luminescence turn-on assay for PPi ions was realized. Utilizing the ability of Cu2+ to differentiate between PPi and Pi, a convenient and straightforward luminescence assay for ALP activity was accomplished based on the specific dephosphorylation of PPi to Pi. To the best of our knowledge, this elaborate luminescence sensing system constitutes the first luminescent nanohybrid material based on a europium organic complex for ALP activity assay. Furthermore, the recognition process of PPi and ALP was completed in a convenient and facile mix-and-readout manner, and it revealed significant potential in point of care testing.
作为磷酸盐代谢中普遍存在的水解酶，碱性磷酸酶（ALP）是实验室研究和临床诊断中的重要生物标志物。在此，我们报告了一种高水溶性的 Eu(DPA)3@Lap 纳米杂化材料，通过发光关闭-开启识别过程，可用于快速和选择性测定焦磷酸盐（PPi）和碱性磷酸酶（ALP）。Eu(DPA)3@Lap 成功在水溶液中制备，具有强发光发射、高光稳定性和长寿命。更有趣的是，由于 DPA 配体与 Cu2+ 离子之间的强配位作用，Eu(DPA)3@Lap 的强发光可以被 Cu2+ 显著猝灭。利用 Cu2+ 作为信号转导物，由于形成了 Cu2+-PPi 络合物，添加 PPi 离子后可以恢复发光，从而实现了对 PPi 离子的发光开启测定。利用 Cu2+ 区分 PPi 和 Pi 的能力，基于 PPi 特异性去磷酸化为 Pi，实现了一种方便快捷的用于测定 ALP 活性的发光测定法。据我们所知，这种精巧的发光传感系统构成了第一个基于镧系元素有机配合物的用于 ALP 活性测定的发光纳米杂化材料。此外，PPi 和 ALP 的识别过程以方便易用的混合-读取方式完成，在即时检测方面显示出了巨大的潜力。
