School of Marine and Biological Engineering, Yancheng Teachers University (YCTU), Yancheng 224002, China.
Department of Basic Medical Science, Jiangsu Vocational College of Medicine, Yancheng 224005, China.
Biosensors (Basel). 2022 Aug 22;12(8):668. doi: 10.3390/bios12080668.
A desirable lanthanide-based ratiometric fluorescence probe was designed as a multifunctional nanoplatform for the determination of dipicolinic acid (DPA), a unique bacterial endospore biomarker, with high selectivity and sensitivity. The carbon dots (CDs) with blue emission wavelengths at 470 nm are developed with europium ion (Eu) to form Eu/CDs fluorescent probes. DPA can specifically combine with Eu and then transfer energy from DPA to Eu sequentially through the antenna effect, resulting in a distinct increase in the red fluorescence emission peak at 615 nm. The fluorescence intensity ratio of Eu/CDs (fluorescence intensity at 615 nm/fluorescence intensity at 470 nm) showed good linearity and low detection limit. The developed ratiometric nanoplatform possesses great potential for application in complex matrices owing to its specificity for DPA. In addition, the integration of a smartphone with the Color Picker APP installed enabled point-of-care testing (POCT) with quantitative measurement capabilities, confirming the great potential of the as-prepared measurement platform for on-site testing.
一种理想的基于镧系元素的比率荧光探针被设计为一种多功能纳米平台，用于测定二吡啶甲酸（DPA），这是一种独特的细菌芽孢生物标志物，具有高选择性和灵敏度。具有 470nm 蓝色发射波长的碳点（CDs）与铕离子（Eu）结合形成 Eu/CDs 荧光探针。DPA 可以特异性地与 Eu 结合，然后通过天线效应将能量从 DPA 依次传递到 Eu，导致在 615nm 处的红色荧光发射峰明显增加。Eu/CDs 的荧光强度比（615nm 处的荧光强度/470nm 处的荧光强度）表现出良好的线性和低检测限。由于该比率纳米平台对 DPA 具有特异性，因此它具有在复杂基质中应用的巨大潜力。此外，将智能手机与安装的 Color Picker APP 集成在一起，实现了具有定量测量能力的即时检测（POCT），证实了所制备的测量平台在现场测试中的巨大潜力。
