Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China.
Mol Med Rep. 2018 Jun;17(6):7737-7745. doi: 10.3892/mmr.2018.8832. Epub 2018 Mar 29.
Strabismus is a common ocular disorder in children and may result in exterior abnormalities and impaired visual functions. However, the detailed pathogenesis of strabismus unclear. The present study assessed the comprehensive analyses on the roles of RNAs in the development of strabismus. The public datasets of strabismus and the corresponding control tissues were downloaded from the Gene Expression Omnibus (GEO). Reannotations of the dysregulated coding and long non‑coding RNAs (lncRNAs) and functional enrichments of the differently expressed genes (DEGs) were conducted. A total of 790 DEGs were screened (648 upregulated and 142 downregulated) in the present study. Among the DEGs, a total of 32 differently expressed lncRNAs were detected (14 upregulated and 18 downregulated). When the Gene Ontology (GO) enrichment was considered, it was identified that a total of 143 GO terms (82 for biological process, 31 for cellular component and 30 for molecular function) were identified. Among all the 57 detected Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, the phagosome pathway, which was labeled as hsa004145, demonstrated the most bioinformatics importance. However, most lncRNAs, except LINC01279 and LOC643733, indicated <3 target mRNAs and were not suitable for advanced bioinformatics analyses. Bioinformatics analyses demonstrated that there was a GO term for each lncRNA (proteinaceous extracellular for LINC01279 and cell surface for LOC643733). In conclusion, a set of coding RNA as well as lncRNAs differentially expressed in strabismus EOM samples were indicated. Notably, the present findings important information for advanced pathogenesis research and biomarkers detection.
斜视是儿童常见的眼部疾病，可导致外观异常和视觉功能受损。然而，斜视的确切发病机制尚不清楚。本研究评估了 RNA 在斜视发展中的作用的综合分析。从基因表达综合数据库（GEO）下载了斜视的公共数据集和相应的对照组织。对失调编码和长非编码 RNA（lncRNA）进行了重新注释，并对差异表达基因（DEG）进行了功能富集分析。本研究共筛选出 790 个 DEG（648 个上调和 142 个下调）。在这些 DEG 中，共检测到 32 个差异表达的 lncRNA（14 个上调和 18 个下调）。当考虑基因本体论（GO）富集时，共鉴定出 143 个 GO 术语（82 个用于生物学过程，31 个用于细胞成分，30 个用于分子功能）。在所检测的 57 个京都基因与基因组百科全书（KEGG）通路中，吞噬体通路（hsa004145）最重要。然而，大多数 lncRNA，除了 LINC01279 和 LOC643733，其对应的靶 mRNA 数量均<3，不适合进行高级生物信息学分析。生物信息学分析表明，每个 lncRNA 都有一个 GO 术语（LINC01279 为蛋白细胞外，LOC643733 为细胞表面）。总之，本研究提示了斜视眼外肌样本中一组差异表达的编码 RNA 和 lncRNA。这些发现为斜视的发病机制研究和生物标志物检测提供了重要信息。
