Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
Liaoning Provincial Key Laboratory for Agricultural Biotechnology, College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, Liaoning 110866, P.R. China.
Int J Mol Med. 2018 Jul;42(1):53-60. doi: 10.3892/ijmm.2018.3602. Epub 2018 Mar 30.
In vitro production of functional spermatids has special significance in the research of spermatogenesis and the treatment of male infertility. Primordial germ cells (PGCs) are the precursors of oocyte and sperm, which generate the totipotent cells. Studies have shown that PGCs have the potential ability to develop meiotic spermatids in vitro. Here we have shown that retinoic acid (RA) leads to PGC differentiation, and SCF can improve the efficiency of induction. We indicate an efficient approach to produce haploid spermatids from chicken PGCs in the presence of RA and stem cell factor (SCF). Real-time RT-PCR assays showed that RA and SCF induced a remarkable increase in expression of SYCP1, ACR, BOULE and DCM1 of meiotic germ cells and haploid germ cells, respectively. DNA content assays revealed that RA and SCF induced a remarkable increase of haploid cells. This study provides a theoretical basis and a great animal model for spermatogenesis study.
体外产生功能性精子在精子发生研究和男性不育治疗中具有特殊意义。原始生殖细胞（PGC）是卵母细胞和精子的前体，可产生全能细胞。研究表明，PGC 具有在体外发育减数分裂精子的潜在能力。在这里，我们表明视黄酸（RA）可导致 PGC 分化，而干细胞因子（SCF）可提高诱导效率。我们提出了一种有效的方法，即在 RA 和干细胞因子（SCF）的存在下从鸡 PGC 中产生单倍体精子。实时 RT-PCR 分析表明，RA 和 SCF 分别诱导减数分裂生殖细胞和单倍体生殖细胞中 SYCP1、ACR、BOULE 和 DCM1 的表达显著增加。DNA 含量分析表明，RA 和 SCF 诱导了单倍体细胞的显著增加。这项研究为精子发生研究提供了理论基础和良好的动物模型。
