Jin Kai, Zhou Jing, Zuo Qisheng, Song Jiuzhou, Zhang Yani, Chang Guobing, Chen Guohong, Li Bichun
Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2020 Nov 27;10(12):2228. doi: 10.3390/ani10122228.
Chicken () pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes.
鸡的多能胚胎干细胞（ESCs）和原始生殖细胞（PGCs）可广泛应用于发育生物学和胚胎学研究，但两性ESCs和PGCs之间的差异仍不明确。本研究通过聚合酶链反应（PCR）和荧光激活细胞分选仪（FACS）鉴定特定的性别标记来确定所收集样本的性别。利用RNA测序（RNA-seq）研究鸡两性ESCs和PGCs的转录组图谱。结果显示，两性ESCs中无显著差异表达基因（DEGs），而两性PGCs中有227个DEGs。此外，根据京都基因与基因组百科全书（KEGG）分析，这227个DEGs主要富集在17个基因本体（GO）术语和27条通路中。此外，进行了qRT-PCR以验证RNA-seq结果，结果表明Notch1在雄性PGCs中高表达。总之，我们的结果提供了鸡两性ESCs和PGCs的知识库，有助于未来相关生物学过程的研究。
