Xu Lijian, Xie Shaowen, Du Jingjing, He Nongyue
J Nanosci Nanotechnol. 2017 Jan;17(1):238-43. doi: 10.1166/jnn.2017.12441.
A novel and sensitive electrochemical sensor based on magnetic porous pseudo-carbon paste electrode (MPPCPE) for DNA detection was described in this study. The MPPCPE was fabricated by mixing polymethyl methacrylate (PMMA) microspheres, as template, graphite powders, as filler, pyrrole, as precursor of polymer and magnetic powder. It was demonstrated that the fabricated MPPCPE had more sensitivity for detecting DNA when compared with magnetic carbon paste electrode (MPCPE). Results from anodic stripping voltammetry (ASV) experiments showed that the Ag peak current versus concentration of complementary DNA at MPPCPE had two linear regions. The first region demonstrated linearity over a concentration range from 0.5 to 20 nM, with correlation coefficient of 0.992, while the second linear region had concentration of complementary DNA at 0.005 to 0.1 nM range, with correlation coefficient of 0.957. The detection limit was as low as 0.005 nM and the electrochemical sensor was easy to preserve for a long time, and had good reusability and stability.
本研究描述了一种基于磁性多孔伪碳糊电极（MPPCPE）的新型灵敏电化学传感器用于DNA检测。MPPCPE是通过将聚甲基丙烯酸甲酯（PMMA）微球作为模板、石墨粉作为填料、吡咯作为聚合物前体以及磁粉混合制备而成。结果表明，与磁性碳糊电极（MPCPE）相比，制备的MPPCPE对DNA检测具有更高的灵敏度。阳极溶出伏安法（ASV）实验结果表明，MPPCPE上的Ag峰电流与互补DNA浓度有两个线性区域。第一个区域在0.5至20 nM的浓度范围内呈线性，相关系数为0.992，而第二个线性区域的互补DNA浓度在0.005至0.1 nM范围内，相关系数为0.957。检测限低至0.005 nM，该电化学传感器易于长期保存，具有良好的可重复使用性和稳定性。
