L-谷氨酸电化学生物传感器的制备及电化学行为

Preparation and electrochemical behavior of L-glutamate electrochemical biosensor.

机构信息

Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China.

出版信息

J Biomed Nanotechnol. 2013 Feb;9(2):318-21. doi: 10.1166/jbn.2013.1487.

DOI:10.1166/jbn.2013.1487

PMID:23627063

Abstract

Using the nano-porous pseudo carbon paste electrode (Nano-PPCPE) as the working electrode, mixing L-glutamate oxidase (L-GLOD), catalase (Cat) and bovine serum albumin (BSA) with phosphate buffer (PB, pH = 7.4), followed by cross-linking with glutaraldehyde, a novel L-glutamate electrochemical biosensor was successfully formed. It was demonstrated that the modified nano-PPCPE exhibits a high selectivity and sensitivity in comparison with the modified CPE. The L-glutamate biosensor showed a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M.

摘要

使用纳米多孔伪碳糊电极（Nano-PPCPE）作为工作电极，将 L-谷氨酸氧化酶（L-GLOD）、过氧化氢酶（Cat）和牛血清白蛋白（BSA）与磷酸盐缓冲液（PB，pH = 7.4）混合，然后用戊二醛交联，成功制备了新型 L-谷氨酸电化学生物传感器。与修饰后的 CPE 相比，修饰后的纳米-PPCPE 表现出更高的选择性和灵敏度。L-谷氨酸生物传感器的线性范围为 5 x 10(-7) M 至 1 x 10(-5) M，检测限为 2.5 x 10(-7) M。