不同磷酸钙氟化物清漆在乳牙上氟释放的3个月研究

Rirattanapong Praphasri, Vongsavan Kadkao, Saengsirinavin Chavengkiat, Jantarakam Nisarat

Southeast Asian J Trop Med Public Health. 2016 Sep;47(5):1098-104.

A range of dental varnishes containing several calcium and phosphate compounds in addition to fluoride to promote remineralization have recently been commercialized. However, the fluoride varnish in the presence of calcium and phosphate can react to form relative poorly soluble phases and its bioavailability. Most of previous studies have focused on fluoride release over a short period. The purpose of this in vitro study was to evaluate the fluoride release from different fluoride varnishes on primary teeth during 3 months. Twenty-five sound primary incisors were randomly divided into five groups: Group A-control group (no treatment), Group B - 5% sodium fluoride varnish (5%NaF)(Duraphat®), Group C-5% sodium fluoride plus tricalcium phosphate varnish (5%NaF+TCP)(ClinproTM White), Group D- 5% sodium fluoride plus amorphous calcium phosphate varnish (5%NaF+ACP)(Enamel Pro®), and Group E-5% sodium fluoride plus tricalcium phosphate varnish (5%NaF+TCP)(Mahidol). The samples were then immersed in artificial saliva at room temperature until used. The concentration of fluoride released was measured with F-ion-specific electrode at 2, 4, 8, 12, 24, and 48 hours and then weekly for three months. To analyze the results, we used the one-way ANOVA and Tukey’s multiple comparison tests at a 95% level of confidence. Group E had the greatest initial fluoride release within the first 24 hours and Group B had the lowest initial release fluoride of the treatment groups. Group B had a slower rate of decline in fluoride release over time than the other treatment groups. By 3 months, the varnishes with the highest to the lowest release of fluoride were Group B=C>E>D>A. All the treatment samples released more fluoride than the control group. Duraphat®(5%NaF) and Clinprotm(5%NaF+TCP) had the highest release of fluoride at 3-month evaluation. The TCP fluoride varnish released more fluoride than the ACP fluoride varnish by 3 months.

最近，一系列除氟化物外还含有多种钙和磷化合物以促进再矿化的牙科用清漆已实现商业化。然而，在钙和磷存在的情况下，氟化物清漆会发生反应形成相对难溶的相及其生物利用度。以前的大多数研究都集中在短时间内的氟化物释放上。这项体外研究的目的是评估不同氟化物清漆在3个月内从乳牙中释放氟化物的情况。将25颗完好的乳切牙随机分为五组：A组为对照组（未处理），B组为5%氟化钠清漆（5%NaF）（Duraphat®），C组为5%氟化钠加磷酸三钙清漆（5%NaF+TCP）（ClinproTM White），D组为5%氟化钠加无定形磷酸钙清漆（5%NaF+ACP）（Enamel Pro®），E组为5%氟化钠加磷酸三钙清漆（5%NaF+TCP）（Mahidol）。然后将样本在室温下浸泡在人工唾液中备用。在2、4、8、12、24和48小时，然后在三个月内每周用氟离子特异性电极测量释放的氟化物浓度。为了分析结果，我们使用了95%置信水平的单因素方差分析和Tukey多重比较检验。E组在最初24小时内的氟化物释放量最大，B组在治疗组中最初的氟化物释放量最低。随着时间的推移，B组氟化物释放量的下降速度比其他治疗组慢。到3个月时，氟化物释放量从高到低的清漆组依次为B=C>E>D>A。所有处理样本释放的氟化物都比对照组多。在3个月评估时，Duraphat®（5%NaF）和Clinprotm（5%NaF+TCP）的氟化物释放量最高。到3个月时，TCP氟化物清漆释放的氟化物比ACP氟化物清漆多。