Food Safety Laboratory, Division of Metrology in Chemistry, National Institute of Metrology, Beijing 100029, China.
Food Safety Laboratory, Division of Metrology in Chemistry, National Institute of Metrology, Beijing 100029, China.
Food Chem. 2018 Aug 15;257:1-6. doi: 10.1016/j.foodchem.2018.02.077. Epub 2018 Feb 14.
Quick and simple analytical methodology has been developed for the measurement of patulin in apple juice by combining single-drop liquid-liquid-liquid microextraction with isotope dilution ultra-high performance liquid chromatography-mass spectrometry. After systematic parameters optimization, the whole sample pretreatment only consisted of single extraction that consumed 1.5 mL ethyl acetate. While, other methods usually needed complicated pretreatment, including extraction, purification, evaporation and redissolution. This sample pretreatment method could greatly lower the interferences from sugar-rich matrix, and the limit of detection was 0.5 μg/L and limit of quantification was 2 μg/L in apple juice. Moreover, linear range covered three orders of magnitude from 2 to 2000 μg/L. The proposed method is promising for patulin extraction and detection in apple juice, which will opens a new perspective in the enrichment of trace contaminations in high sugar complex matrix.
建立了一种快速、简单的分析方法，用于通过单滴液-液-液微萃取结合同位素稀释超高效液相色谱-质谱法测定苹果汁中的棒曲霉素。在系统地优化参数后，整个样品预处理仅包括消耗 1.5 mL 乙酸乙酯的单次萃取。而其他方法通常需要复杂的预处理，包括提取、净化、蒸发和重溶解。这种样品预处理方法可以大大降低富含糖的基质的干扰，在苹果汁中的检测限为 0.5μg/L，定量限为 2μg/L。此外，线性范围涵盖了从 2 到 2000μg/L 的三个数量级。该方法有望用于苹果汁中棒曲霉素的提取和检测，为高糖复杂基质中痕量污染物的富集开辟了新的视角。
