基于生物素-亲和素系统的灵敏适体传感器用于苹果汁中棒曲霉素的检测。
A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice.
机构信息
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.
出版信息
Biosensors (Basel). 2022 Jan 23;12(2):59. doi: 10.3390/bios12020059.
Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7139.8 ng/mL in the buffer and 7.07359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B (AFB), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice.
展青霉素污染的水果、蔬菜及其制品被认为是一个严重的健康风险因素的食品安全和人类健康。因此，快速、简单的检测方法对于展青霉素变得重要，它可以为常规筛选和食品调查提供一个工具。本研究的目的是开发一种灵敏的适配体为基础的侧向流动测定法（FLA）使用链霉亲和素功能化金纳米粒子用于灵敏的展青霉素检测。获得了展青霉素检测的一个极好的动态范围（2.7139.8ng/ml在缓冲液和7.07359.5ng/ml在样品）没有亲和力为其他真菌毒素如玉米赤霉烯酮（ZEN）、赭曲霉毒素 A（OTA）、黄曲霉毒素 B（AFB）、桔霉素或 tenuazonic 酸（TEA）。检测限为 0.19ng/ml 在缓冲液和 0.36ng/ml 在实际样品中。回收率为 83.3%至 107.1%，从 6.5%到 7.5%满意的 RSD 值。因此，建立的 LFA 可作为一种快速、简单、现场筛选工具用于果汁中的苹果汁中的 PAT 测定。
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