Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Food Chem. 2018 Aug 15;257:211-215. doi: 10.1016/j.foodchem.2018.03.020. Epub 2018 Mar 8.
Cytotoxicity and genotoxicity of sodium acetate (SA), sodium diacetate (SDA), and potassium sorbate (PS) was tested on Human Umbilical Vein Endothelial Cells (HUVEC). Cytotoxicity was investigated by MTT assay and flow cytometry analysis, while genotoxicity was evaluated using DNA fragmentation and DAPI staining assays. The growth of treated HUVECs with various concentrations of SA, SDA and PS decreased in a dose-and time-dependent manner. The IC50 of 487.71, 485.82 and 659.96 µM after 24 h and IC50 of 232.05, 190.19 and 123.95 µM after 48 h of treatment were attained for SA, SDA and PS, respectively. Flow cytometry analysis showed that early and late apoptosis percentage in treated cells was not considerable. Also neither considerable DNA fragmentation nor DNA smear was observed using DAPI staining and DNA ladder assays. Overall, it can be concluded that the aforementioned food additives can be used as safe additives at low concentration in food industry.
我们测试了醋酸钠（SA）、双乙酸钠（SDA）和山梨酸钾（PS）对人脐静脉内皮细胞（HUVEC）的细胞毒性和遗传毒性。通过 MTT 检测和流式细胞术分析来研究细胞毒性，而通过 DNA 片段化和 DAPI 染色检测来评估遗传毒性。用不同浓度的 SA、SDA 和 PS 处理的 HUVEC 的生长呈剂量和时间依赖性下降。处理 24 小时后，SA、SDA 和 PS 的 IC50 值分别为 487.71、485.82 和 659.96µM，处理 48 小时后，SA、SDA 和 PS 的 IC50 值分别为 232.05、190.19 和 123.95µM。流式细胞术分析表明，处理细胞的早期和晚期凋亡百分比并不显著。同时，DAPI 染色和 DNA 梯状电泳检测也未观察到明显的 DNA 片段化或 DNA 弥散。总的来说，可以得出结论，上述食品添加剂在食品工业中可以作为安全添加剂在低浓度下使用。
