Schulze M, Bortfeldt R, Schäfer J, Jung M, Fuchs-Kittowski F
Institute for the Reproduction of Farm Animals Schönow Inc., Bernauer Allee 10, D-16321 Bernau, Germany.
Institute for the Reproduction of Farm Animals Schönow Inc., Bernauer Allee 10, D-16321 Bernau, Germany.
Anim Reprod Sci. 2018 May;192:328-334. doi: 10.1016/j.anireprosci.2018.03.035. Epub 2018 Mar 31.
The shipping of semen doses to sow farms can impair boar semen quality. Unfortunately, there is currently no practice-oriented information available regarding general shipping conditions of boar semen. For this reason, a special mobile sensing app (TransportLog 1.0), utilizing the built-in sensors of smartphones, has been programmed to capture vibration emissions during shipping of semen doses (QuickTip Flexitubes®, Minitüb). Data were analyzed, transformed and used as standards for simulating vibration emissions from an orbital shaker IKA MTS 4 (Laborgeräte München) in a spermatological reference laboratory. Twenty ejaculates were collected randomly and diluted using a one-step isothermal process in a split-sample procedure in Beltsville Thawing Solution (BTS, Minitüb). The sperm concentration was adjusted to 24 × 10 sperm/mL. The dose filling volume was 85 ± 1 mL. Samples were stored for seven days at 17 °C. The results showed that circular horizontal vibration emissions with frequencies of 300 rpm for a duration of 6 h led to a significant alkalization of the BTS-extended semen. Semen motility, mitochondrial activity, acrosome and plasma membrane integrity as well as thermo-resistance all demonstrated a frequency-dependent negative response to vibration emissions during long-term storage. This study leads to new insights and recommendations for the shipping of boar semen in the artificial insemination industry. Furthermore, a new monitoring tool for boar semen shipping was established using mobile sensing.
向母猪场运送精液剂量可能会损害公猪精液质量。不幸的是，目前尚无关于公猪精液一般运输条件的面向实际操作的信息。因此，已编写了一款特殊的移动传感应用程序（TransportLog 1.0），利用智能手机的内置传感器来捕捉精液剂量（QuickTip Flexitubes®，Minitüb）运输过程中的振动排放。在一个精子学参考实验室中，对数据进行了分析、转换，并用作模拟IKA MTS 4（慕尼黑实验室设备公司）轨道振荡器振动排放的标准。随机采集了20份射精样本，并在贝尔茨维尔解冻液（BTS，Minitüb）中采用分步样本程序通过一步等温过程进行稀释。将精子浓度调整至24×10⁶精子/mL。剂量填充体积为85±1 mL。样本在17°C下储存7天。结果表明，频率为300 rpm、持续6小时的圆形水平振动排放导致BTS稀释精液显著碱化。在长期储存期间，精液活力、线粒体活性、顶体和质膜完整性以及耐热性均对振动排放表现出频率依赖性负面反应。本研究为人工授精行业中公猪精液的运输带来了新的见解和建议。此外，利用移动传感建立了一种用于公猪精液运输的新监测工具。
