Hafemeister Tim, Schulze Paul, Simmet Christian, Jung Markus, Fuchs-Kittowski Frank, Schulze Martin
Institute for Reproduction of Farm Animals Schönow, 16321 Bernau, Germany.
Environmental Computer Science, HTW Berlin-University of Applied Sciences, Wilhelminenhofstr. 75a, 12459 Berlin, Germany.
Animals (Basel). 2023 Mar 6;13(5):952. doi: 10.3390/ani13050952.
Vibration emissions during the transport of boar semen for artificial insemination (AI) affect sperm quality. In the present study, the common influence of the following factors was investigated: vibrations (displacement index (D) = 0.5 to 6.0), duration of transport (0 to 12 h) and storage time (days 1 to 4). Normospermic ejaculates were collected from 39 fertile Pietrain boars (aged 18.6 ± 4.5 months) and diluted in a one-step procedure with an isothermic (32 °C) BTS (Minitüb) extender ( = 546 samples). Sperm concentration was adjusted to 22 × 10 sperm·mL. Extended semen (85 ± 1 mL) was filled into 95 mL QuickTip Flexitubes (Minitüb). For transport simulation on day 0, a laboratory shaker IKA MTS 4 was used. Total sperm motility (TSM) was evaluated on days 1 to 4. Thermo-resistance test (TRT), mitochondrial activity (MITO) and plasma membrane integrity (PMI) were assessed on day 4. Sperm quality dropped with increasing vibration intensity and transport duration, and the effect was enhanced by a longer storage time. A linear regression was performed using a mixed model, accounting for the boar as a random effect. The interaction between D and transport duration significantly ( < 0.001) explained data for TSM (-0.30 ± 0.03%), TRT (-0.39 ± 0.06%), MITO (-0.45 ± 0.06%) and PMI (-0.43 ± 0.05%). Additionally, TSM decreased by 0.66 ± 0.08% with each day of storage ( < 0.001). It can be concluded that boar semen extended in BTS should be transported carefully. If this is not possible or the semen doses are transported a long way, the storage time should be reduced to a minimum.
用于人工授精（AI）的公猪精液运输过程中的振动排放会影响精子质量。在本研究中，调查了以下因素的共同影响：振动（位移指数（D）= 0.5至6.0）、运输持续时间（0至12小时）和储存时间（第1至4天）。从39头育肥皮特兰公猪（年龄18.6±4.5个月）采集正常精子的射精，并在等温（32°C）的BTS（Minitüb）稀释剂中进行一步稀释程序（n = 546个样本）。将精子浓度调整至22×10⁶精子·mL⁻¹。将稀释后的精液（85±1 mL）装入95 mL QuickTip Flexitubes（Minitüb）中。在第0天使用实验室振荡器IKA MTS 4进行运输模拟。在第1至4天评估总精子活力（TSM）。在第4天评估耐热性测试（TRT）、线粒体活性（MITO）和质膜完整性（PMI）。精子质量随着振动强度和运输持续时间的增加而下降，并且储存时间越长，这种影响越明显。使用混合模型进行线性回归，将公猪作为随机效应。D与运输持续时间之间的相互作用显著（P < 0.001）解释了TSM（-0.30±0.03%）、TRT（-0.39±0.06%）、MITO（-0.45±0.06%）和PMI（-0.43±0.05%）的数据。此外，储存每一天TSM下降0.66±0.08%（P < 0.001）。可以得出结论，用BTS稀释的公猪精液应小心运输。如果无法做到这一点或精液剂量运输距离较长，则应将储存时间减至最短。
