Shou Kangquan, Qu Chunrong, Sun Yao, Chen Hao, Chen Si, Zhang Lei, Xu Haibo, Hong Xuechuan, Yu Aixi, Cheng Zhen
Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China.
Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, 1201 Welch Rd, Lucas P095, Stanford, CA 94305-5484, USA.
Adv Funct Mater. 2017 Jun 20;27(23). doi: 10.1002/adfm.201700995. Epub 2017 Apr 24.
Compared with imaging in the visible (400 - 650 nm) and near-infrared window I (NIR-I, 650 - 900 nm) regions, imaging in near-infrared window II (NIR-II, 1,000-1,700 nm) is a highly promising imaging modality with improved resolution and deeper tissue penetration. In this work, a small molecule NIR-II dye,5,5'-(1H,5H-benzo[1,2-c:4,5-c'] bis[1,2,5]thiadiazole)-4,8-diyl)bis(N,N-bis(4-(3-((tert-butyldimethylsilyl)oxy)propyl)phenyl) thiophen-2-amine), has been successfully encapsulated into phospholipid vesicles to prepare a probe CQS1000. Then this novel NIR-II probe has been studied for multifunctional biological imaging. Our results indicate that the NIR-II vesicle CQS1000 can noninvasively and dynamically visualize and monitor many physiological and pathological conditions of circulatory systems, including lymphatic drainage and routing, angiogenesis of tumor and vascular deformity such as arterial thrombus formation and ischemia with high spatial and temporal resolution. More importantly, by virtue of the favorable half-life of blood circulation of CQS1000, NIR-II imaging is capable of aiding us to accomplish precise resection of tumor such as osteosarcoma, and to accelerate the process of lymph nodes dissection to complete sentinel lymph node biopsy for better decision-making during the tumor surgery. Overall, CQS1000 is a highly promising NIR-II probe for multifunctional biomedical imaging in physiological and pathological conditions, surpassing traditional NIR-I imaging modality and pathologic assessments for clinical diagnosis and treatment.
与在可见光（400 - 650纳米）和近红外窗口I（NIR-I，650 - 900纳米）区域的成像相比，近红外窗口II（NIR-II，1000 - 1700纳米）成像具有更高的分辨率和更深的组织穿透能力，是一种极具前景的成像方式。在这项工作中，一种小分子NIR-II染料，5,5'-（1H,5H-苯并[1,2-c:4,5-c']双[1,2,5]噻二唑）-4,8-二基）双（N,N-双（4-（3-（（叔丁基二甲基甲硅烷基）氧基）丙基）苯基）噻吩-2-胺），已成功封装到磷脂囊泡中，制备出探针CQS1000。然后对这种新型NIR-II探针进行了多功能生物成像研究。我们的结果表明，NIR-II囊泡CQS1000可以无创、动态地可视化和监测循环系统的许多生理和病理状况，包括淋巴引流和路径、肿瘤血管生成以及血管畸形，如动脉血栓形成和缺血，具有高空间和时间分辨率。更重要的是，凭借CQS1000良好的血液循环半衰期，NIR-II成像能够帮助我们完成骨肉瘤等肿瘤的精确切除，并加速淋巴结清扫过程，以完成前哨淋巴结活检，从而在肿瘤手术中做出更好的决策。总体而言，CQS1000是一种极具前景的NIR-II探针，可用于生理和病理状况下的多功能生物医学成像，超越了传统的NIR-I成像方式和病理评估，用于临床诊断和治疗。
