Foundation for Research and Technology-Hellas (F.O.R.T.H.), Institute of Electronic Structure and Laser (I.E.S.L.), Heraklion, Greece.
Biomater Sci. 2018 May 29;6(6):1469-1479. doi: 10.1039/c7bm00904f.
In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogeneously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e. oligopeptides, small molecules and oligomers) were successfully attached onto the microstructures. Experiments with PC12 cells on hierarchical substrates incorporating AuNPs carrying the RGD peptide showed an impressive growth and NGF-induced differentiation of the PC12 cells, compared to that on the NP-free, bare, micropatterned substrates. The exploitation of the developed methodology for the binding of AuNPs as carriers of specific bio-functional moieties onto micropatterned culture substrates for cell biology studies is envisaged.
在这项工作中，我们报告了一种开发包含功能化金纳米粒子（AuNPs）的分层细胞培养平台的新方法。特别是，分层基底包括初级硅微锥的伪周期性阵列，与具有生物功能基团的均匀沉积 AuNPs 的二次纳米级图案相结合。具有各种功能的 AuNPs（即寡肽、小分子和低聚物）成功地附着在微结构上。在含有携带 RGD 肽的 AuNPs 的分层基底上进行的 PC12 细胞实验表明，与无 NP、裸露、微图案化基底相比，PC12 细胞的生长和 NGF 诱导的分化令人印象深刻。预计将开发的方法用于将 AuNPs 作为特定生物功能基团的载体结合到用于细胞生物学研究的微图案化培养基底上。
