Mochimaru Yuta, Negishi Jun, Murakami Syo, Musha Shiori, Sato Koichi, Okajima Fumikazu, Tomura Hideaki
1 Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan.
2 Laboratory of Medical Neuroscience, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan.
Zoolog Sci. 2018 Apr;35(2):109-114. doi: 10.2108/zs170145.
Human, mouse, and zebrafish ovarian cancer G protein-coupled receptors (OGR1s) are activated by both metals and extracellular protons. In the present study, we examined whether pig, rat, chicken, and Xenopus OGR1 homologs could sense and be activated by protons and metals. We found that all homologs stimulated serum response element (SRE)-driven promoter activities when they are stimulated by protons. On the other hand, metals differentially activated the homologs. The results using chimeric receptors of human and zebrafish OGR1s indicate that the specificity of the metal-induced activation lies in the extracellular region. These results suggest that protons are an evolutionally conserved agonist of OGR1. However, the types of metals that activated the receptor differed among the homologs.
人类、小鼠和斑马鱼的卵巢癌G蛋白偶联受体（OGR1s）可被金属离子和细胞外质子激活。在本研究中，我们检测了猪、大鼠、鸡和非洲爪蟾的OGR1同源物是否能感知质子和金属离子并被其激活。我们发现，所有同源物在受到质子刺激时都会刺激血清反应元件（SRE）驱动的启动子活性。另一方面，金属离子对同源物的激活存在差异。使用人类和斑马鱼OGR1嵌合受体的结果表明，金属离子诱导激活的特异性位于细胞外区域。这些结果表明，质子是OGR1在进化上保守的激动剂。然而，激活该受体的金属离子类型在不同同源物之间存在差异。
