Murakami Syo, Mochimaru Yuta, Musha Shiori, Kojima Ryotaro, Deai Masahito, Mogi Chihiro, Sato Koichi, Okajima Fumikazu, Tomura Hideaki
Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan.
Laboratory of Integrated Signaling Systems, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan.
Zoolog Sci. 2020 Apr;37(2):103-108. doi: 10.2108/zs190106.
Ogerin is a positive allosteric modulator of human and mouse ovarian cancer G protein-coupled receptors (OGR1s). In the present study, we found that ogerin differentially enhances the activation of OGR1 in various animal species. Amino acid residues of OGR1 that are associated with ogerin are conserved among the species. This suggests that other amino acid residues may be involved in the action of ogerin. Chimeric receptors between human and zebrafish OGR1s showed that the amino acid residues that determine the species specificity of ogerin-induced enhancement reside in the transmembrane and/or intracellular regions of OGR1. This result highlights the importance of first verifying the effectiveness of ogerin to the OGR1 of the species of interest at the cellular level prior to analyzing the physiological and pathophysiological roles of OGR1 in the species.
奥杰林是人和小鼠卵巢癌G蛋白偶联受体（OGR1）的正向变构调节剂。在本研究中，我们发现奥杰林在不同动物物种中对OGR1激活的增强作用存在差异。与奥杰林相关的OGR1氨基酸残基在物种间是保守的。这表明其他氨基酸残基可能参与了奥杰林的作用。人和斑马鱼OGR1之间的嵌合受体表明，决定奥杰林诱导增强的物种特异性的氨基酸残基位于OGR1的跨膜和/或细胞内区域。这一结果凸显了在分析OGR1在某物种中的生理和病理生理作用之前，先在细胞水平验证奥杰林对该物种OGR1有效性的重要性。
