Moon Eun-Kyung, Lee Seungeun, Quan Fu-Shi, Kong Hyun-Hee
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, Republic of Korea.
Department of Parasitology, Dong-A University College of Medicine, Busan 49201, Republic of Korea.
Exp Parasitol. 2018 May;188:102-106. doi: 10.1016/j.exppara.2018.04.005. Epub 2018 Apr 3.
Acanthamoeba keratitis is commonly encountered by contact lens wearers. Contact lens solution plays an important role in the safe use of contact lenses. The most popular products for disinfecting lenses are multipurpose disinfecting solutions (MPDS). However, almost all MPDS retailed in Korea are ineffective in killing Acanthamoeba. The objective of this study was to determine the possibility of using autophagy inhibitor chloroquine as a disinfecting agent to improve the amoebicidal activity of MPDS against Acanthamoeba, especially the cyst. Amoebicidal effects of eight different MPDSs combined with chloroquine (CQ), an autophagy inhibitor, and their cytotoxicities to human corneal epithelium cells were determined. Almost all MPDS showed strong amoebicidal effect on trophozoites after 8 h of exposure. However, they showed inadequate amoebicidal effect on cysts even after 24 h of exposure. MPDSs combined with 100 μM CQ increased their amoebicidal effects on immature cyst by inhibiting formation of mature cysts. Incubation with 100 μM CQ for 30 min did not have cytotoxicity to human corneal epithelial cells.
棘阿米巴角膜炎在隐形眼镜佩戴者中较为常见。隐形眼镜护理液在隐形眼镜的安全使用中起着重要作用。最常用的镜片消毒产品是多功能护理液（MPDS）。然而，韩国零售的几乎所有MPDS对杀灭棘阿米巴均无效。本研究的目的是确定使用自噬抑制剂氯喹作为消毒剂以提高MPDS对棘阿米巴尤其是包囊的杀阿米巴活性的可能性。测定了八种不同的MPDS与自噬抑制剂氯喹（CQ）联合使用时的杀阿米巴效果及其对人角膜上皮细胞的细胞毒性。几乎所有MPDS在暴露8小时后对滋养体均显示出较强的杀阿米巴作用。然而，即使在暴露24小时后，它们对包囊的杀阿米巴作用仍不足。与100μM CQ联合使用的MPDS通过抑制成熟包囊的形成增强了对未成熟包囊的杀阿米巴作用。用100μM CQ孵育30分钟对人角膜上皮细胞无细胞毒性。
