Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.
Invest Ophthalmol Vis Sci. 2013 Jul 5;54(7):4527-37. doi: 10.1167/iovs.13-11927.
To investigate experimental variables in the development of standardized methods to assess the efficacy of contact lens disinfection systems against the trophozoite and cysts of Acanthamoeba spp.
A. castellanii (ATCC 50370), A. polyphaga (ATCC 30461), and A. hatchetti (CDC: V573) were adapted to axenic culture and used to produce cysts either with Neff's encystment medium (NEM) or starvation on nonnutrient agar (NNA). Challenge test assays and a most probable number approach were used to compare the trophozoite and cysticidal efficacy of four multipurpose disinfectant solutions (MPDSs) and a one-step hydrogen peroxide system (with and without the neutralizing step).
With trophozoites, four of four MPDSs and the one-step peroxide system gave ≥3 log₁₀ kill for all strains 6 hours, regardless of culture medium used. Greater resistance was found against cysts, with results for MPDSs varying by species and method of cyst production. Here, 1-3 log₁₀ kill was found with NEM cysts for three of four MPDSs compared with one of four for the NNA cysts at 6 hours (A. castellanii and A. polyphaga, only). The one-step peroxide system gave 1-1.9 log₁₀ kill with NEM cysts and 0.8-1.1 for NNA cysts. Only 3% hydrogen peroxide gave total kill (>3 log₁₀) of NNA cysts at 6 hours.
A reproducible method for determining the susceptibility of Acanthamoeba trophozoites and cysts to contact lens care systems has been developed. This will facilitate assay standardization for assessing the efficacy of such products against the organism and aid development of improved disinfectant and therapeutic agents.
研究评估接触镜消毒系统对棘阿米巴滋养体和包囊疗效的标准化方法中实验变量。
适应于无细胞培养的棘阿米巴属（Acanthamoeba）的棘阿米巴属（Acanthamoeba）castellanii（ATCC 50370）、棘阿米巴属（Acanthamoeba）polyphaga（ATCC 30461）和棘阿米巴属（Acanthamoeba）hatchetti（CDC：V573），并用 Neff 包囊培养基（NEM）或非营养琼脂（NNA）饥饿法产生包囊。采用挑战试验和最可能数方法比较了四种多功能消毒剂（MPDS）和一种一步法过氧化氢系统（有和无中和步骤）对滋养体和囊杀效果。
用滋养体，四种 MPDS 和一步法过氧系统在 6 小时内对所有菌株的杀灭率均≥3log₁₀，与使用的培养基无关。包囊对结果的抵抗力更强，MPDS 的结果因物种和包囊产生方法而异。在这里，与 NNA 包囊相比，四种 MPDS 中有三种在 6 小时时对 NEM 包囊的杀灭率为 1-3log₁₀（只有棘阿米巴属和棘阿米巴属属）。一步法过氧系统对 NEM 包囊的杀灭率为 1-1.9log₁₀，对 NNA 包囊的杀灭率为 0.8-1.1。只有 3%的过氧化氢在 6 小时时对 NNA 包囊进行了总杀灭（>3log₁₀）。
已经开发出一种用于确定棘阿米巴滋养体和包囊对接触镜护理系统敏感性的可重复方法。这将有助于评估此类产品对该生物体疗效的检测标准化，并有助于开发更好的消毒剂和治疗剂。
