Mukhtiar Muhammad, Jan Syed Umer, IhsanUllah -, Gul Rahman, Hussain Abid, Ali Essa, IzharUllah -, Jabbar Abdul, Akram Muhammad, Mansoor Sibghat, Khan Muhammad Farid
Department of Pharmacy, Faculty of Medical & Health Science, The University of Poonch. Rawalakot, Pakistan.
Faculty of Pharmacy and Health Sciences, University of Balochistan, Quetta, Pakistan.
Pak J Pharm Sci. 2018 Mar;31(2(Suppl.)):727-731.
Glutathione is an essential antioxidant of living organism that provides a primary protection against metals toxicity. A significant amount of glutathione is present in blood erythrocytes, plasma and liver hepatocytes to protect them from oxidative damage from both external and internal oxidants. Metalo-element palladium has numerous pharmacological, clinical and toxicological compensations, like palladium is used as anti-viral, anti-bacterial, neuro-protective and anti-tumor agent. However studies have also indicated some mild to serious toxic effects of palladium metallo-elements. In the presence study the interaction of palladium inorganic salt and organic complex with glutathione (GSH) content of liver homogenate was examined spectro-photometrically. 20% (w/v) liver homogenate was prepared of the collected liver of rabbit in 5% TCA (tri-chloro-acetic acid) solution and 1mm EDTA, using a potter-eveljhem homogenizer with motor driven Teflon pestle. The GSH content quantification was carried out by Elman's method. Our finding showed that there was a depletion of GSH content by both palladium inorganic salts and organic complexes, concentrations wise as well as with time elapse as level of GSH content decrease from (43.6% to 72.62%) with Palladium Nitrate and from (24.09 to 59.5%) with Bis-benzonitrile Palladium II Chloride as compared to control, and further dropped with time incubation from 0-90 minutes from (49.7 to 87.1%), with Palladium Nitrate and from (29.3% to 67.6%) respectively. The result showed that the effect of both inorganic salt of palladium was more enhanced as compare to its organic complex. It was suggested from our finding that the depletion in the glutathione content of liver homogenate may be due to oxidation of glutathione or due to glutathione metal abduct formation by both inorganic salt and organic complex of palladium. This study in situ is a model of in vivo.
谷胱甘肽是生物体必需的抗氧化剂，可提供针对金属毒性的主要保护作用。血液红细胞、血浆和肝脏肝细胞中存在大量谷胱甘肽，以保护它们免受外部和内部氧化剂的氧化损伤。金属元素钯具有多种药理、临床和毒理学作用，例如钯被用作抗病毒、抗菌、神经保护和抗肿瘤剂。然而，研究也表明钯金属元素存在一些轻度至严重的毒性作用。在本研究中，采用分光光度法检测了钯无机盐和有机配合物与肝脏匀浆中谷胱甘肽（GSH）含量的相互作用。使用带有电动聚四氟乙烯杵的波特-埃尔维耶姆匀浆器，在5%三氯乙酸（TCA）溶液和1mM乙二胺四乙酸（EDTA）中，从收集的兔肝脏制备20%（w/v）肝脏匀浆。通过埃尔曼法进行GSH含量定量。我们的研究结果表明，钯无机盐和有机配合物都会导致GSH含量减少，从浓度和时间上看，与对照组相比，硝酸钯使GSH含量水平从（43.6%降至72.62%），二氯双苄腈钯（II）使GSH含量从（24.09降至59.5%），并且在0至90分钟的孵育时间内进一步下降，硝酸钯从（49.7%降至87.1%），二氯双苄腈钯（II）分别从（29.3%降至67.6%）。结果表明，钯的无机盐的作用比其有机配合物更强。我们的研究结果表明，肝脏匀浆中谷胱甘肽含量的减少可能是由于谷胱甘肽的氧化，或者是由于钯的无机盐和有机配合物形成了谷胱甘肽金属结合物。本原位研究是体内模型。
