Khan Muhammad Jalil, Mukhtiar Muhammad, Qureshi Muhammad Murtaza, Jan Syed Umer, IzharUllah -, Hussain Abid, Khan Muhammad Farid, Gul Rahman, Shahwani Nisar Ahmad, Rabbani Imran
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gomal University, Dera Ismail Khan, Pakistan.
Department of Pharmacy, The University of Poonch, Rawalakot. AJK, Pakistan.
Pak J Pharm Sci. 2018 Jul;31(4(Supplementary)):1463-1467.
Thallium has been shown to significantly influence various tissues of living organisms; Exposure to Thallium can disturb mitochondrial function, degenerate neurons, and interfere with the function of critical metabolic enzymes and co-enzymes. Glutathione (GSH) an essential biomarker is considered a key factor in harnessing the thallium toxicity. In the present study the interaction of Thallium (Thallium Chloride) and glutathione was investigated spectro-photo-metrically in aqueous media. The renowned Elman's experimental protocol was followed at a wavelength of 412nm for Glutathione quantification in each sample. The pH of each sample was maintained at 7.6 using Phosphate buffer during the entire course of the experiment. A concentration as well as time dependent depletion of glutathione after exposure to various concentration of Thallium metal was observed, revealing chemical interaction between the metal and glutathione. The exact mechanism of interaction of Thallium and glutathione is still to be investigated. However, this piece of research suggests that a decrease in the concentration of Glutathione may be due to Thallium-GSH abduct or oxidize glutathione (GSSG) formation. This study was performed in-vitro as a model of in vivo.
铊已被证明会对生物体的各种组织产生显著影响；接触铊会干扰线粒体功能、使神经元退化，并干扰关键代谢酶和辅酶的功能。谷胱甘肽（GSH）作为一种重要的生物标志物，被认为是控制铊毒性的关键因素。在本研究中，采用分光光度法研究了铊（氯化铊）与谷胱甘肽在水介质中的相互作用。在412nm波长下，按照著名的埃尔曼实验方案对每个样品中的谷胱甘肽进行定量。在整个实验过程中，使用磷酸盐缓冲液将每个样品的pH值保持在7.6。观察到暴露于不同浓度的铊金属后，谷胱甘肽的浓度和时间依赖性消耗，揭示了金属与谷胱甘肽之间的化学相互作用。铊与谷胱甘肽相互作用的确切机制仍有待研究。然而，这项研究表明，谷胱甘肽浓度的降低可能是由于铊-谷胱甘肽结合物或氧化型谷胱甘肽（GSSG）的形成。本研究作为体内模型在体外进行。
