Justus C, Müller S, Kramer M D
Ernst-Rodenwaldt Institut, Fachbereich Veterinärmedizin, Mainz, F.R.G.
Br J Dermatol. 1987 Dec;117(6):687-94. doi: 10.1111/j.1365-2133.1987.tb07347.x.
To study the organization of the plasminogen activator/plasminogen-plasmin (PA/PG-P) system in human epidermis we raised monoclonal antibodies with specificity for human plasminogen and plasmin (PG-P). Monoclonal antibody P2, which appeared most suitable for immunohistology, is a mouse monoclonal antibody of IgG1 subtype, specific for the precursor enzyme plasminogen and for the high molecular weight chain of the active enzyme, plasmin. Immunofluorescence analysis of normal human epidermis revealed that reactivity with P2 was confined to the basal cell layer. In psoriatic lesions, however, this regional organization was not found. Immunoreactivity in this case was scattered throughout all the hyperproliferating cell layers, which is taken as evidence for an altered distribution of PG-P in psoriatic lesions. In psoriasis other components of the PA/PG-P system have previously been found to be altered. In this context our findings add to the hypothesis that this system may be involved in the pathology or the pathogenesis of psoriatic lesions.
为了研究人表皮中纤溶酶原激活物/纤溶酶原 - 纤溶酶（PA/PG - P）系统的组织情况，我们制备了对人纤溶酶原和纤溶酶（PG - P）具有特异性的单克隆抗体。单克隆抗体P2似乎最适用于免疫组织学研究，它是一种IgG1亚型的小鼠单克隆抗体，对前体酶纤溶酶原以及活性酶纤溶酶的高分子量链具有特异性。对正常人表皮进行免疫荧光分析发现，与P2的反应性局限于基底细胞层。然而，在银屑病皮损中，未发现这种区域组织分布情况。在这种情况下，免疫反应性分散在所有增殖活跃的细胞层中，这被视为PG - P在银屑病皮损中分布改变的证据。在银屑病中，先前已发现PA/PG - P系统的其他成分发生了改变。在此背景下，我们的研究结果进一步支持了该系统可能参与银屑病皮损病理或发病机制的假说。
