Isseroff R R, Rifkin D B
J Invest Dermatol. 1983 Apr;80(4):297-9. doi: 10.1111/1523-1747.ep12534677.
This study was undertaken to determine whether plasminogen was present within the epidermis. Cryostat sections of normal human skin were incubated with either whole rabbit antihuman plasminogen serum, the IgG fraction thereof, or the immune-specific IgG fraction thereof, purified by affinity chromatography on plasminogen-Sepharose. Standard indirect immunofluorescent techniques using rhodamine-conjugated goat antirabbit IgG were subsequently employed. Fluorescence was localized to the basal layer of the epidermis. Nonimmune serum and immune serum depleted of its specific anti-plasminogen IgG by prior affinity chromatography yielded negative results, demonstrating the specificity of staining for plasminogen. Our findings indicate that plasminogen is present within the epidermis, localized to the peripheral intracytoplasmic area of basal keratinocytes. This finding supports the hypothesis that a proteolytic event mediated by plasminogen activator with the subsequent generation of plasmin may be involved in normal keratinocyte physiology.
本研究旨在确定表皮内是否存在纤溶酶原。将正常人皮肤的低温切片与全兔抗人纤溶酶原血清、其IgG组分或经纤溶酶原-琼脂糖亲和层析纯化的免疫特异性IgG组分一起孵育。随后采用使用罗丹明偶联的山羊抗兔IgG的标准间接免疫荧光技术。荧光定位于表皮的基底层。预先通过亲和层析去除其特异性抗纤溶酶原IgG的非免疫血清和免疫血清产生阴性结果，证明了纤溶酶原染色的特异性。我们的研究结果表明，纤溶酶原存在于表皮内，定位于基底角质形成细胞的外周胞质区域。这一发现支持了这样一种假说，即由纤溶酶原激活剂介导的蛋白水解事件以及随后纤溶酶的产生可能参与正常角质形成细胞的生理过程。
