Chen Yajun, Yu Miao, Hemandez J A, Li Jiexi, Yuan Zi-Guo, Yan Haikuo
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, PR China; Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases of Guangdong Province, South China Agricultural University, 510642, PR China.
Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, 510140, PR China.
Exp Parasitol. 2018 May;188:73-78. doi: 10.1016/j.exppara.2018.04.003. Epub 2018 Apr 4.
We constructed a new plasmid pIRESneo/ROP18/PLP1 that was injected intramuscularly into Kunming mice to evaluate its immune efficacy. The immunized mice exhibited significantly increased serum IgG2a levels, lymphocyte counts and Th1-type cytokine (IL-2, IL-12 and IFN-γ) levels. Moreover, the immunized mice exhibited longer survival times (44.7 ± 2.1 days for ROP18/PLP1 and 47.2 ± 2.9 days for ROP18/PLP1 + IL-18) and lower brain cyst burden (68.9% for ROP18/PLP1 and 72.4% for ROP18/PLP1 + IL-18) than control mice after T. gondii challenge. Our results demonstrate that the multiple-gene DNA vaccine including both ROP18 and PLP1 elicits greater protection against T. gondii challenge and stronger immunogenicity than single-gene vaccines.
我们构建了一种新的质粒pIRESneo/ROP18/PLP1，并将其肌肉注射到昆明小鼠体内以评估其免疫效果。免疫后的小鼠血清IgG2a水平、淋巴细胞计数和Th1型细胞因子（IL-2、IL-12和IFN-γ）水平显著升高。此外，与弓形虫攻击后的对照小鼠相比，免疫后的小鼠存活时间更长（ROP18/PLP1组为44.7±2.1天，ROP18/PLP1 + IL-18组为47.2±2.9天），脑囊肿负担更低（ROP18/PLP1组为68.9%，ROP18/PLP1 + IL-18组为72.4%）。我们的结果表明，包含ROP18和PLP1的多基因DNA疫苗比单基因疫苗对弓形虫攻击具有更强的保护作用和免疫原性。
