Shin Jun-Young, Ji Jong-Ok, Choi Da-Woon, Choi Sang-Heon, Choi Jong-Gu, Rho Min-Suk, Lee Ji Yoon, Lee Sang-Suk
Department of Oriental Biomedical Engineering, Sangji University, Wonju, Republic of Korea.
Goodpl Co., Ltd, Wonju, Republic of Korea.
J Acupunct Meridian Stud. 2019 Feb;12(1):3-10. doi: 10.1016/j.jams.2018.03.003. Epub 2018 Apr 4.
It is known that the primo vascular system (PVS) includes the primo nodes and vessels. However, the relevant genes in the PVS system for both pathologic and physiologic condition are poorly understood. Here, we first examined the gene expression in primo vessels (PVs) floating in lymphatic endothelium by isolation of PVS and lymphatic vessels (LVs) containing PVS. To investigate therapeutic effects, both PVs and LVs containing PVS were isolated after lipopolysaccharide injection and acupuncture electric stimulation at two acupoints Joksamni (ST36) and Hapgok (LI04) following lipopolysaccharide injection. We used reverse transcriptase-polymerase chain reaction to examine expression of lymphatic endothelial cell markers and inflammatory related genes. We found that lymphatic endothelial cell markers such as fms-related tyrosine kinase 4 (Flt4), lymphatic vessel endothelial receptor (Lyve-1), prospero homeobox protein 1 (Prox-1), and podoplanin (Pdpn) were highly expressed in PV compared to that of lymphatic endothelium, suggesting pivotal roles of PV in LV under inflammation. Furthermore, lymphatic-related genes including metal-response element-binding transcription factor 2 (Mtf2), hypoxia inducible factor (Hif1a), angiotensin II type 1 receptor (Agtr1), and angiotensin II type 2 receptor (Agtr2) were also overall increased in PV, and remarkably increased and these genes except peroxisome proliferator-activated receptor gamma (Pparg) after acupuncture electric stimulation in two acupoints implying central role of PV by gene activation.
已知初级血管系统（PVS）包括初级节点和血管。然而，对于PVS系统中病理和生理状态下的相关基因，我们了解甚少。在此，我们首先通过分离PVS和含有PVS的淋巴管（LVs），检测漂浮在淋巴管内皮中的初级血管（PVs）中的基因表达。为了研究治疗效果，在注射脂多糖后，于两个穴位足三里（ST36）和合谷（LI04）进行针刺电刺激，然后分离含有PVS的PVs和LVs。我们使用逆转录聚合酶链反应来检测淋巴管内皮细胞标志物和炎症相关基因的表达。我们发现，与淋巴管内皮相比，初级血管中淋巴管内皮细胞标志物如fms相关酪氨酸激酶4（Flt4）、淋巴管内皮受体（Lyve-1）、prospero同源盒蛋白1（Prox-1）和血小板内皮细胞黏附分子（Pdpn）高度表达，这表明在炎症状态下初级血管在淋巴管中起关键作用。此外，包括金属反应元件结合转录因子2（Mtf2）、缺氧诱导因子（Hif1a）、血管紧张素II 1型受体（Agtr1）和血管紧张素II 2型受体（Agtr2）在内的淋巴管相关基因在初级血管中总体也有所增加，并且在两个穴位进行针刺电刺激后，除过氧化物酶体增殖物激活受体γ（Pparg）外这些基因显著增加，这意味着通过基因激活初级血管发挥核心作用。
