Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
Department of Animal Biotechnology, College of Biology Sciences, Sangji University, Wonju, Republic of Korea.
Adv Exp Med Biol. 2021;1269:387-391. doi: 10.1007/978-3-030-48238-1_61.
The gene expression of Prox-1 and Hif-1a for the isolated primo vessels (PVs) and composite lymphatic vessels (LVs) containing PVs (LVs + PVs) was investigated by RNA-sequencing (Seq) and quantitative polymerase chain reaction (qRT-PCR) analysis. RNA-Seq on the passed 10 samples on RNA-QC for two experimental groups with PVs and PVs + LVs proceeded to the library construction stage automatically and analyzed differentially expressed genes (DEGs). From the real-time qRT-PCR analysis data, we found the marker genes of Prox-1 and Hif-1a were enriched and decreased in an isolated PVs compared to LVs, respectively. Based on mRNA transcriptional data, Prox-1 and Hif-1a were increased and decreased in PVs compared to LVs + PVs under lipopolysaccharide (LPS) treatment and relieved by acupuncture electric stimulation (AES), respectively. This finding indicates that high and low levels of Prox-1 and Hif-1a may be involved in the function of PVs and that pathophysiological and physiological condition could progress into inflamed lymphatic endothelial cells expanding the PV within the LV.
通过 RNA 测序 (Seq) 和定量聚合酶链反应 (qRT-PCR) 分析，研究了分离的初级血管 (PVs) 和包含 PVs (LVs + PVs) 的复合淋巴管 (LVs) 的 Prox-1 和 Hif-1a 的基因表达。对于包含 PVs 和 PVs + LVs 的两个实验组的 10 个经过 RNA-QC 的样本进行了 RNA-Seq，自动进入文库构建阶段，并分析差异表达基因 (DEGs)。从实时 qRT-PCR 分析数据中，我们发现与 LVs 相比，Prox-1 和 Hif-1a 的标记基因在分离的 PVs 中分别富集和减少。基于 mRNA 转录数据，在 LPS 处理下，与 LVs + PVs 相比，Prox-1 和 Hif-1a 在 PVs 中分别增加和减少，而电针刺激 (AES) 可缓解这种情况。这一发现表明，高和低水平的 Prox-1 和 Hif-1a 可能参与了 PVs 的功能，并且病理生理和生理状况可能会导致炎症性淋巴管内皮细胞扩张，使 LV 内的 PV 扩张。
