Ottoila P, Taskinen J
Orion Corporation Ltd, Orion Pharmaceutica, Espoo, Finland.
Biomed Environ Mass Spectrom. 1987 Nov;14(11):659-62. doi: 10.1002/bms.1200141116.
A method for simultaneous quantitative determination of erythromycin 2'-ethylsuccinate and erythromycin in human plasma is described. After extracting the deuterium-labelled internal standards and analytes from alkalinized plasma to diethyl ether the extracts were analysed by fast atom bombardment mass spectrometry. The quantification limit for 2'-ethylsuccinate ester was 50 ng ml-1 and for erythromycin 100 ng ml-1. The calibration curves were linear up to 5 micrograms ml-1 for both. The precision of the method at higher concentration was less than 2% and at the quantification limit approximately 6% for both analytes.
本文描述了一种同时定量测定人血浆中琥乙红霉素和红霉素的方法。从碱化血浆中用乙醚提取氘标记的内标和分析物后，提取物通过快原子轰击质谱法进行分析。琥乙红霉素酯的定量限为50 ng/ml，红霉素为100 ng/ml。两者的校准曲线在高达5μg/ml时呈线性。该方法在较高浓度下的精密度对于两种分析物均小于2%，在定量限时约为6%。
