Tsuji K
J Chromatogr. 1978 Oct 1;158:337-48. doi: 10.1016/s0021-9673(00)89978-1.
The method described is capable of detecting less than 0.01 microgram/ml of erythromycin and/or erythromycin ethylsuccinate in serum. Recoveries of erythromycin and erythromycin ethylsuccinate, when added to the level of 0.6 microgram/ml, were 102 and 97% with relative standard deviations of 6.0 and 4.5%, respectively. Erythromycin ethylsuccinate in serum at 37 degrees was shown to degrade rapidly to erythromycin in a first-order rate. When stored at -20 degrees, however, only 10% of the erythromycin ethylsuccinate in serum was hydrolyzed even after storage for 36 days. The method was used to analyze erythromycin and erythromycin ethylsuccinate in sera from ten subjects administered with an oral dose of erythromycin ethylsuccinate. In addition to a small amount of anhydroerythromycin ethylsuccinate and 8,9-anhydro-6,9-hemiketal erythromycin ethylsuccinate, at least two other metabolites were detected in sera and one was tentatively identified as erythralosamine.
所述方法能够检测血清中低于0.01微克/毫升的红霉素和/或琥乙红霉素。当添加至0.6微克/毫升水平时，红霉素和琥乙红霉素的回收率分别为102%和97%，相对标准偏差分别为6.0%和4.5%。血清中的琥乙红霉素在37℃时显示以一级速率迅速降解为红霉素。然而，当储存在-20℃时，即使储存36天后，血清中只有10%的琥乙红霉素被水解。该方法用于分析10名口服琥乙红霉素受试者血清中的红霉素和琥乙红霉素。除了少量脱水琥乙红霉素和8,9-脱水-6,9-半缩酮琥乙红霉素外，在血清中还检测到至少两种其他代谢物，其中一种初步鉴定为红霉糖胺。
