Faculty of Pharmacy, Fujian University of Traditional Chinese Medicine, Minhao, Fuzhou, Fujian Province, China.
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jun 15;199:271-282. doi: 10.1016/j.saa.2018.03.061. Epub 2018 Mar 24.
Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae) is a precious traditional Chinese medicinal herb and has been perennially used to treat various illness. However, there were unethical sellers who adulterated wild A. roxburghii with tissue cultured and cultivated ones. Therefore, there is an urgent need for an effective authentication method to differentiate between these different types of A. roxburghii. In this research, the infrared spectroscopic tri-step identification approach including Fourier transform infrared spectroscopy (FT-IR), Second derivative infrared spectra (SD-IR) and two-dimensional correlation infrared spectra (2D-IR) was used to develop a simple and rapid method to discriminate between wild, cultivated and tissue cultivated A. roxburghii plant. Through this study, all three types of A. roxburghii plant were successfully identified and discriminated through the infrared spectroscopic tri-step identification method. Besides that, all the samples of wild, cultivated and tissue cultivated A. roxburghii plant were analysed with the Soft Independent Modelling of Class Analogy (SIMCA) pattern recognition technique to test and verify the experimental results. The results showed that the three types of A. roxburghii can be discriminated clearly as the recognition rate was 100% for all three types and the rejection rate was more than 60%. 70% of the validated samples were also identified correctly by the SIMCA model. The SIMCA model was also validated by comparing 70 standard herbs to the model. As a result, it was demonstrated that the macroscopic IR fingerprint method and the classification analysis could discriminate not only between the A. roxburghi samples and the standard herbs, it could also distinguish between the three different types of A. roxburghi plant in a direct, rapid and holistic manner.
铁皮石斛（Wall.）林德利（兰科）是一种珍贵的传统中药，长期以来一直用于治疗各种疾病。然而，有不道德的卖家将野生铁皮石斛与组织培养和栽培的铁皮石斛混合。因此，迫切需要一种有效的鉴定方法来区分这些不同类型的铁皮石斛。在这项研究中，采用傅里叶变换红外光谱（FT-IR）、二阶导数红外光谱（SD-IR）和二维相关红外光谱（2D-IR）的红外光谱三步鉴定方法，开发了一种简单快速的方法来区分野生、栽培和组织培养的铁皮石斛植物。通过这项研究，成功地通过红外光谱三步鉴定方法鉴定和区分了所有三种类型的铁皮石斛植物。此外，所有野生、栽培和组织培养的铁皮石斛植物样本均采用软独立建模分类分析（SIMCA）模式识别技术进行分析，以检验和验证实验结果。结果表明，三种类型的铁皮石斛可以清晰地区分，因为三种类型的识别率均为 100%，拒绝率均高于 60%。SIMCA 模型也正确识别了 70%的验证样本。通过将 70 种标准药材与模型进行比较，也验证了 SIMCA 模型。结果表明，宏观红外指纹方法和分类分析不仅可以区分铁皮石斛样品和标准药材，还可以直接、快速、全面地区分三种不同类型的铁皮石斛植物。
