College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Mechanical and Electronic Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Molecules. 2020 Jan 2;25(1):193. doi: 10.3390/molecules25010193.
The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant . Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenoside, while response surface method (RSM) was used to optimize the extraction process. The best possible range for methanol concentration (0-100%), the liquid-solid ratio (5:1-30:1 mL/g), ultrasonic power (240-540 W), duration of ultrasound (10-50 min), ultrasonic temperature (10-60 °C), and the number of extractions (1-4) were obtained according to the single factor experiments. Then, using the Box-Behnken design (BBD) of response surface analysis, the optimum extraction conditions were obtained with 16.33% methanol concentration, the liquid-solid ratio of 10.83:1 mL/g and 35.00 °C ultrasonic temperature. Under these conditions, kinsenoside extraction yield reached 32.24% dry weight. The best conditions were applied to determine the kinsenoside content in seven different cultivation ages in .
本研究旨在建立一种从全株中提取金雀异黄素化合物的方法。采用超声辅助提取（UAE）和超高效液相色谱（UPLC）法提取并测定金雀异黄素的含量，同时采用响应面法（RSM）对提取工艺进行优化。根据单因素实验，得到甲醇浓度（0-100%）、液固比（5:1-30:1 mL/g）、超声功率（240-540 W）、超声时间（10-50 min）、超声温度（10-60°C）和提取次数（1-4）的最佳可能范围。然后，利用响应面分析的 Box-Behnken 设计（BBD），得到最佳提取条件为甲醇浓度 16.33%、液固比 10.83:1 mL/g 和超声温度 35.00°C。在此条件下，金雀异黄素提取率达到 32.24%干重。将最佳条件应用于测定不同培养年龄的 7 种中的金雀异黄素含量。
