State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, People's Republic of China; Guizhou Chemical Drug Research and Development Engineering Technical Center, Guiyang 550004, People's Republic of China.
Guizhou Chemical Drug Research and Development Engineering Technical Center, Guiyang 550004, People's Republic of China.
Pestic Biochem Physiol. 2018 Apr;146:13-18. doi: 10.1016/j.pestbp.2018.02.001. Epub 2018 Feb 6.
A series of limonoids (1-8) were isolated from the whole plant of Munronia henryi and antiviral activities of the compounds were evaluated. The bioassay results demonstrated that Munronin O (1) showed remarkable protective activity and compounds 7 and 8 showed significant inactivating, protective, and curative activities against tobacco mosaic virus (TMV). With a 50% effective concentration (EC) value of 91.5 μg/mL, compound 1 exhibited the best protective activity compared with ningnanmycin (192.3 μg/mL). The potential for these compound of inducing systemic acquired resistance (SAR) was also evaluated, and compound 1 showed excellent induction activities. Furthermore, it was found that potentiation of defense-related enzyme activity and the contents of SA was increased. Compound 1 could also inhibit the expression of TMV CP and up-regulate the expression of defense-related genes. This work revealed that compound 1 can induce resistance and enhance plant tolerance to TMV infection. Hence, compound 1 can be considered as a potential activator for inducing plant resistance.
从蒙椴全株中分离得到一系列柠檬苦素类化合物（1-8），并评价了它们的抗病毒活性。生物测定结果表明，蒙椴宁 O（1）对烟草花叶病毒（TMV）具有显著的保护活性，化合物 7 和 8 对 TMV 具有显著的灭活、保护和治疗活性。化合物 1 的 50%有效浓度（EC）值为 91.5μg/mL，与宁南霉素（192.3μg/mL）相比，其具有最佳的保护活性。还评估了这些化合物诱导系统获得抗性（SAR）的潜力，发现化合物 1 具有优异的诱导活性。此外，发现防御相关酶活性和 SA 含量增加。化合物 1 还可以抑制 TMV CP 的表达并上调防御相关基因的表达。这项工作表明，化合物 1 可以诱导抗性并增强植物对 TMV 感染的耐受性。因此，化合物 1 可被视为一种潜在的诱导植物抗性的激活剂。
