Castellanos Mario R, Nehru Vijeyaluxmy Motilal, Pirog Edyta C, Optiz Lynne
Division of Research, Department of Medicine, Staten Island University Hospital - Northwell Health, 475 Seaview Ave, Staten Island, NY 10305, USA.
Department of Pathology, Weill Cornell Medical College, 525 East 68th Street, New York, NY 10065, USA.
Pathol Res Pract. 2018 May;214(5):605-611. doi: 10.1016/j.prp.2018.03.021. Epub 2018 Mar 26.
Prevention of cervical cancer is based upon the accurate diagnosis and grading of cervical lesions identified during screening. The pathological classification of cervical intraepithelial neoplasia (CIN) is problematic, as it relies on subjective criteria and is known to have high interobserver variability and low reproducibility. These limitations can result in either over or under treatment of patients. Biomarkers to improve CIN diagnosis have not overcome all these challenges.
Here we review the use of a promising optical imaging method using eosin-based fluorescence spectroscopy. This technique is able to perform fluorescent analysis of cervical biopsies directly from hematoxylin and eosin (H&E) stained tissues. Eosin is a brominated derivative of fluorescein. Fluorescence characteristics of protein-eosin complexes can demonstrate tissue changes associated with dysplasia and cancer. In this article we review the progress made towards developing eosin-based fluorescence spectroscopy. We describe the various morphologies seen among the CIN grades with this optical method and highlight the progress made to quantitate the spectral image characteristics.
Eosin-based fluorescence spectroscopy can be used to directly examine H&E stained tissue slides. Relevant areas can be imaged and spectral analysis done to obtain objective data to identify and grade cervical lesions.
宫颈癌的预防基于筛查过程中对宫颈病变的准确诊断和分级。宫颈上皮内瘤变（CIN）的病理分类存在问题，因为它依赖主观标准，且已知具有较高的观察者间变异性和较低的可重复性。这些局限性可能导致患者接受过度或不足的治疗。用于改善CIN诊断的生物标志物尚未克服所有这些挑战。
在此，我们回顾一种有前景的基于曙红的荧光光谱光学成像方法的应用。该技术能够直接对苏木精和伊红（H&E）染色的组织进行宫颈活检的荧光分析。曙红是荧光素的溴化衍生物。蛋白质 - 曙红复合物的荧光特性可显示与发育异常和癌症相关的组织变化。在本文中，我们回顾了基于曙红的荧光光谱技术的发展进展。我们描述了用这种光学方法在不同CIN分级中观察到的各种形态，并强调了在定量光谱图像特征方面取得的进展。
基于曙红的荧光光谱可用于直接检查H&E染色的组织切片。可以对相关区域进行成像并进行光谱分析，以获得客观数据来识别和分级宫颈病变。
