State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China; Shanghai Laboratory Animal Research Center, Shanghai, People's Republic of China.
State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
Vaccine. 2018 May 3;36(19):2581-2588. doi: 10.1016/j.vaccine.2018.03.079. Epub 2018 Apr 5.
Vaccine development efforts against Streptococcus suis serotype 2 (S. suis 2) are often constrained by strain/serotype antigen variability. Bioinformatics analyses revealed two highly conserved S. suis 2 factors, EF-Tu and FtsZ. Murine immunization with recombinant proteins emulsified in white oil adjuvant or eukaryotic DNA vaccine vectors provided significant protection against lethal S. suis 2 challenge. Immune responses elicited by recombinant protein immunization revealed the robust generation of humoral immune responses, with a mixed induction of Th1-type and Th2-type responses. Furthermore, the antiserum from mice immunized with recombinant proteins significantly inhibited the growth of S. suis 2 in healthy pig whole blood, suggesting the triggering of a strong opsonizing response. Histological examination found that immunizing mice with purified recombinant proteins reduced neutrophil and macrophage accumulation in brain and lung tissues after challenge with virulent S. suis. Taken together, these findings reveal that EF-Tu and FtsZ may be promising targets for subunit and DNA vaccine candidates against S. suis 2 infection.
针对猪链球菌血清 2 型（S. suis 2）的疫苗开发工作常常受到菌株/血清型抗原变异性的限制。生物信息学分析显示，S. suis 2 存在两种高度保守的因子，EF-Tu 和 FtsZ。用白油佐剂乳化的重组蛋白或真核 DNA 疫苗载体对小鼠进行免疫接种，可显著提供针对致死性 S. suis 2 挑战的保护。重组蛋白免疫接种引起的免疫应答显示出强大的体液免疫应答产生，诱导了 Th1 型和 Th2 型应答的混合。此外，用重组蛋白免疫接种的小鼠抗血清可显著抑制健康猪全血中 S. suis 2 的生长，表明触发了强烈的调理作用。组织学检查发现，用纯化的重组蛋白免疫接种可减少在具有毒力的 S. suis 攻击后大脑和肺部组织中中性粒细胞和巨噬细胞的积聚。总之，这些发现表明 EF-Tu 和 FtsZ 可能是针对 S. suis 2 感染的亚单位和 DNA 疫苗候选物的有前途的靶标。
