Department of Sanitary Engineering, Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand.
School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
Ecotoxicol Environ Saf. 2018 Aug 15;157:318-326. doi: 10.1016/j.ecoenv.2018.03.066. Epub 2018 Apr 6.
Bisphenol A (BPA) is one of the most abundant endocrine-disrupting compounds which is found in the aquatic environment. However, actual knowledge regarding the effect of plant-bacteria interactions on enhancing BPA removal is still lacking. In the present study, Dracaena sanderiana endophytic bacteria interactions were investigated to evaluate the effect of bacterial inoculation on BPA removal under hydroponic conditions. Two plant growth-promoting (PGP) bacterial strains, Bacillus thuringiensis and Pantoea dispersa, which have high BPA tolerance and can utilize BPA for growth, were used as plant inocula. P. dispersa-inoculated plants showed the highest BPA removal efficiency at 92.32 ± 1.23% compared to other inoculated and non-inoculated plants. This was due to a higher population of the endophytic inoculum within the plant tissues which resulted in maintained levels of indole-3-acetic acid (IAA) for the plant's physiological needs and lower levels of reactive oxygen species (ROS). In contrast, B. thuringiensis-inoculated plants had a lower BPA removal efficiency. However, individual B. thuringiensis possessed a significantly higher BPA removal efficiency compared to P. dispersa. This study provides convincing evidence that not all PGP endophytic bacteria-plant interactions could improve the BPA removal efficiency. Different inocula and inoculation times should be investigated before using plant inoculation to enhance phytoremediation.
双酚 A（BPA）是一种在水生环境中含量最丰富的内分泌干扰化合物之一。然而，关于植物-细菌相互作用增强 BPA 去除的实际知识仍然缺乏。在本研究中，研究了龙血树内生细菌的相互作用，以评估在水培条件下细菌接种对 BPA 去除的影响。两种具有高 BPA 耐受性并可利用 BPA 生长的植物促生（PGP）细菌菌株，苏云金芽孢杆菌和泛菌，被用作植物接种物。与其他接种和未接种的植物相比，接种 P. dispersa 的植物对 BPA 的去除效率最高，为 92.32±1.23%。这是由于内生接种物在植物组织内的种群较高，从而维持了植物生理需求所需的吲哚-3-乙酸（IAA）水平，并降低了活性氧（ROS）水平。相比之下，接种苏云金芽孢杆菌的植物对 BPA 的去除效率较低。然而，单个苏云金芽孢杆菌的 BPA 去除效率明显高于 P. dispersa。本研究提供了令人信服的证据，即并非所有 PGP 内生细菌-植物相互作用都能提高 BPA 的去除效率。在使用植物接种来增强植物修复之前，应该研究不同的接种物和接种时间。
