Khaksar Gholamreza, Treesubsuntorn Chairat, Thiravetyan Paitip
School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
Plant Physiol Biochem. 2016 Oct;107:326-336. doi: 10.1016/j.plaphy.2016.06.020. Epub 2016 Jun 23.
Phytoremediation could be a cost-effective, environmentally friendly approach for the treatment of indoor air. However, some drawbacks still dispute the expediency of phytotechnology. Our objectives were to investigate the competency of plant growth-promoting (PGP) endophytic Bacillus cereus ERBP (endophyte root blue pea), isolated from the root of Clitoria ternatea, to colonize and stabilize within Zamioculcas zamiifolia and Euphorbia milii as non-native hosts without causing any disease or stress symptoms. Moreover, the impact of B. cereus ERBP on the natural shoot endophytic community and for the airborne formaldehyde removal capability of non-native hosts was assessed. Non-native Z. zamiifolia was effectively inoculated with B. cereus ERBP through soil as the most efficient method of endophyte inoculation. Denaturing gradient gel electrophoresis profiling of the shoot endophytic community verified the colonization and stability of B. cereus ERBP within its non-native host during a 20-d fumigation period without interfering with the natural shoot endophytic diversity of Z. zamiifolia. B. cereus ERBP conferred full protection to its non-native host against formaldehyde phytotoxicity and enhanced airborne formaldehyde removal of Z. zamiifolia whereas non-inoculated plants suffered from formaldehyde phytotoxicity because their natural shoot endophytic community was detrimentally affected by formaldehyde. In contrast, B. cereus ERBP inoculation into non-native E. milii deteriorated airborne formaldehyde removal of the non-native host (compared to a non-inoculated one) as B. cereus ERBP interfered with natural shoot endophytic community of E. milii, which caused stress symptoms and stimulated ethylene biosynthesis. Non-native host inoculation with PGP B. cereus ERBP could bear potentials and challenges for airborne formaldehyde removal.
植物修复可能是一种经济高效、环境友好的室内空气处理方法。然而，一些缺点仍然对植物技术的适用性提出了质疑。我们的目标是研究从蝶豆根部分离出的促植物生长（PGP）内生蜡样芽孢杆菌ERBP（内生菌根蓝豌豆）在作为非本地宿主的金钱树和虎刺梅内定殖和稳定的能力，且不引起任何疾病或胁迫症状。此外，还评估了蜡样芽孢杆菌ERBP对自然茎内生菌群的影响以及对非本地宿主去除空气中甲醛能力的影响。通过土壤将蜡样芽孢杆菌ERBP有效地接种到非本地的金钱树中，这是内生菌接种最有效的方法。茎内生菌群的变性梯度凝胶电泳图谱验证了蜡样芽孢杆菌ERBP在20天熏蒸期内在其非本地宿主内的定殖和稳定性，且不干扰金钱树自然茎内生菌群的多样性。蜡样芽孢杆菌ERBP为其非本地宿主提供了完全保护，使其免受甲醛的植物毒性，并增强了金钱树对空气中甲醛的去除能力，而未接种的植物则遭受甲醛的植物毒性，因为它们的自然茎内生菌群受到甲醛的不利影响。相比之下，将蜡样芽孢杆菌ERBP接种到非本地虎刺梅中会降低非本地宿主对空气中甲醛的去除能力（与未接种的相比），因为蜡样芽孢杆菌ERBP干扰了虎刺梅的自然茎内生菌群，导致胁迫症状并刺激乙烯生物合成。用促植物生长的蜡样芽孢杆菌ERBP接种非本地宿主在去除空气中甲醛方面可能具有潜力和挑战。
