Laboratorium für Organische Chemie, Department of Chemistry and Applied Biosciences, ETH-Zürich, 8093 Zürich, Switzerland.
Laboratorium für Organische Chemie, Department of Chemistry and Applied Biosciences, ETH-Zürich, 8093 Zürich, Switzerland; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8602, Japan.
Curr Opin Chem Biol. 2018 Oct;46:18-24. doi: 10.1016/j.cbpa.2018.03.014. Epub 2018 Apr 5.
The concept of synthetic fermentation is to 'grow' complex organic molecules in a controlled and predictable manner by combining small molecule building blocks in water-without the need for reagents, enzymes, or organisms. This approach mimics the production of small mixtures of structurally related natural products by living organisms, particularly microbes, under conditions compatible with direct screening of the cultures for biological activity. This review discusses the development and implementation of this concept, its use for the discovery of protease inhibitors, its basis as a chemistry outreach program allowing non-specialists to make and discover new antibiotics, and highlights of related approaches.
合成发酵的概念是通过在水中组合小分子构建块，以可控和可预测的方式“生长”复杂的有机分子 - 而无需试剂、酶或生物体。这种方法模拟了在与直接筛选培养物的生物活性相容的条件下，由生物体（特别是微生物）产生少量结构相关天然产物的混合物。本综述讨论了该概念的发展和实施、其在蛋白酶抑制剂发现方面的应用、作为化学推广计划的基础，使非专业人员能够制造和发现新的抗生素，以及相关方法的要点。
