Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Burn Research Center, Iran University of Medical Science, Tehran, Iran.
Int J Biol Macromol. 2018 Jul 15;114:1248-1256. doi: 10.1016/j.ijbiomac.2018.04.002. Epub 2018 Apr 5.
Electrospun nanofibers have attracted a lot of attention in recent years in tissue engineering applications. In this research, novel polyvinyl alcohol/gelatin/chondroitin sulfate (PVA/GE/Cs) nanofibrous scaffolds using non-carcinogen solvent system via electrospinning technique was evaluated. A solvent system containing water and acetic acid was used as a safe solvent system to obtain a homogenous mixture with suitable solvent properties and finally non-toxic nanofibrous scaffolds. The effect of water to the acetic acid ratio in the solvent system (7:3, 6:4, 5:5, 4:6, 3:7) and also polymer concentration (8, 9, 10w/v %) on nanofibers morphology was investigated. The appropriate flow rate and voltage ranges to obtain uniform and bead-free electrospun scaffold were investigated. Effect of different Cs ratio (0, 10, 15 and 20wt%) on solution properties was evaluated. Influence of Cs ratio on chemical, physical and thermal properties of the electrospun scaffolds was studied. The results of cell toxicity indicated that prepared PVA/GE/Cs scaffolds have no cell toxicity. SEM results demonstrated that L929 mouse fibroblast cells have suitable interaction with scaffold surface and also attached and proliferated well on the prepared substrate after 24 and 48h and also have a potential for using in tissue engineering.
近年来，静电纺丝纳米纤维在组织工程应用中受到了广泛关注。本研究采用非致癌性溶剂体系通过静电纺丝技术制备了新型聚乙烯醇/明胶/硫酸软骨素（PVA/GE/Cs）纳米纤维支架。以水和乙酸组成的溶剂体系作为安全溶剂体系，以获得具有合适溶剂性能的均匀混合物，最终得到无毒的纳米纤维支架。考察了溶剂体系中水与乙酸的比例（7:3、6:4、5:5、4:6、3:7）以及聚合物浓度（8、9、10w/v%）对纳米纤维形态的影响。研究了获得均匀、无珠状静电纺丝支架的适当流速和电压范围。考察了不同硫酸软骨素比例（0、10、15 和 20wt%）对溶液性能的影响。研究了硫酸软骨素比例对静电纺丝支架化学、物理和热性能的影响。细胞毒性结果表明，所制备的 PVA/GE/Cs 支架无细胞毒性。SEM 结果表明，L929 小鼠成纤维细胞与支架表面具有良好的相互作用，在制备的基底上附着和增殖良好，24h 和 48h 后，在组织工程中具有潜在的应用前景。
